DiIC18(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid)
DiIC<sub>18</sub>(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid)
Citas y referencias (11)
Invitrogen™

DiIC18(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid)

La carbocianina lipofílica DiIC18(5)-DS, con fluorescencia de rojo lejano, contiene grupos de sulfonatos para mejorar la solubilidad en agua. EsMás información
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Número de catálogoCantidad
D12730
también denominado D-12730
5 mg
Número de catálogo D12730
también denominado D-12730
Precio (USD)
-
Cantidad:
5 mg
La carbocianina lipofílica DiIC18(5)-DS, con fluorescencia de rojo lejano, contiene grupos de sulfonatos para mejorar la solubilidad en agua. Es débilmente fluorescente en agua, pero muy fluorescente y bastante fotoestable cuando se incorpora a membranas. Los grupos de sulfonatos incorporados en este analógico del DiI mejoran la solubilidad en agua. Tiene un coeficiente de extinción extremadamente alto y tiempos de vida de estado excitado cortos (∼ 1 nanosegundo) en entornos lipídicos. Una vez aplicado a las células, el colorante se difunde lateralmente dentro de la membrana plasmática.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorRojo
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia
Cantidad5 mg
Condiciones de envíoTemperatura ambiente
Tipo de productoDiIC18(5)-DS
SubCellular LocalizationMembrana plasmática, membranas celulares y lípidos, Lipids, Plasma Membrane
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

Lipophilic cyanine dyes are preferred for this sort of assay, since they insert into cellular membranes and then, upon fusion, are shared by the fused cells as the membranes are shared. For example, one cell population can be labeled with DiI (orange-red) and another cell population can be labeled with DiO (green), and when the cells fuse, the combined color appears yellow (when imaged with a dual-bandpass filter set).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (11)

Citations & References
Abstract
Constructing size distributions of liposomes from single-object fluorescence measurements.
Authors:Lohr C, Kunding AH, Bhatia VK, Stamou D,
Journal:Methods Enzymol
PubMed ID:19913166
We describe in detail a simple technique to construct the size distribution of liposome formulations from single-object fluorescence measurements. Liposomes that are fluorescently labeled in their membrane are first immobilized on a surface at dilute densities and then imaged individually using epi-fluorescence microscopy. The integrated intensities of several thousand single ... More
Lipophilic dye staining of Cryptococcus neoformans extracellular vesicles and capsule.
Authors:Nicola AM, Frases S, Casadevall A,
Journal:Eukaryot Cell
PubMed ID:19465562
Cryptococcus neoformans is an encapsulated yeast that causes systemic mycosis in immunosuppressed individuals. Recent studies have determined that this fungus produces vesicles that are released to the extracellular environment both in vivo and in vitro. These vesicles contain assorted cargo that includes several molecules associated with virulence and implicated in ... More
Distribution of liposomes into brain and rat brain tumor models by convection-enhanced delivery monitored with magnetic resonance imaging.
Authors:Saito R, Bringas JR, McKnight TR, Wendland MF, Mamot C, Drummond DC, Kirpotin DB, Park JW, Berger MS, Bankiewicz KS
Journal:Cancer Res
PubMed ID:15059914
Although liposomes have been used as a vehicle for delivery of therapeutic agents in oncology, their efficacy in targeting brain tumors has been limited due to poor penetration through the blood-brain barrier. Because convection-enhanced delivery (CED) of liposomes may improve the therapeutic index for targeting brain tumors, we conducted a ... More
Modular microcircuit organization of the presubicular head-direction map.
Authors:
Journal:Cell Rep
PubMed ID:35417686
Dual HER2 Targeting with Trastuzumab and Liposomal-Encapsulated Doxorubicin (MM-302) Demonstrates Synergistic Antitumor Activity in Breast and Gastric Cancer.
Authors:
Journal:Cancer Res
PubMed ID:26759238
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