DiFMU (6,8-Difluoro-7-Hydroxy-4-Methylcoumarin), reference standard
Citas y referencias (4)
Invitrogen™
DiFMU (6,8-Difluoro-7-Hydroxy-4-Methylcoumarin), reference standard
DIFMU puede usarse como estándar de referencia para los sustratos de éter, éster y fosfato derivados de este colorante azulMás información
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Número de catálogo
Cantidad
D6566
10 mg
Número de catálogo D6566
Precio (USD)
-
Cantidad:
10 mg
DIFMU puede usarse como estándar de referencia para los sustratos de éter, éster y fosfato derivados de este colorante azul fluorescente.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Etiqueta o tinteDiFMU
Tipo de productoEstándar de referencia
Cantidad10 mg
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes de cumarina
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.
Citations & References (4)
Citations & References
Abstract
A fluorogenic substrate for the continuous assaying of aryl sulfatases.
Authors:Ahmed V, Ispahany M, Ruttgaizer S, Guillemette G, Taylor SD,
Journal:Anal Biochem
PubMed ID:15802133
'The most common fluorogenic substrate for assaying aryl sulfatases (ARSs) is 4-methylumbelliferyl sulfate (MUS). However, ARSs operate optimally at pH values that are less than the pK(a) (7.8) of the reaction product of MUS, 4-methylumbelliferone (4-MU). Thus, a major disadvantage of this assay is that it is usually run in ... More
Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes.
Authors:Sun WC, Gee KR, Haugland RP
Journal:Bioorg Med Chem Lett
PubMed ID:9873685
'Two new fluorinated fluorescent dyes, 6,8-difluoro-7-hydroxy-4-methylcoumarin (Marina Blue) and 3-carboxy-6,8-difluoro-7-hydroxycoumarin (Pacific Blue), exhibit excellent photophysical properties among a series of novel fluorinated 7-hydroxycoumarins. Most of these fluorinated coumarins have quantum yields (0.63 to 0.89) equal to or higher than that of the parent compound (0.63), which, in combination with their ... More
Characterization of the histidine-containing phosphotransfer protein B-mediated multistep phosphorelay system in Pseudomonas aeruginosa PAO1.
Authors:Hsu JL, Chen HC, Peng HL, Chang HY,
Journal:J Biol Chem
PubMed ID:18256026
Certain bacterial two-component sensor kinases possess a histidine-containing phosphotransfer (Hpt) domain to carry out a multistep phosphotransferring reaction to a cognate response regulator. Pseudomonas aeruginosa PAO1 contains three genes that encode proteins with an Hpt domain but lack a kinase domain. To identify the sensor kinase coupled to these Hpt ... More
A fluorogenic substrate for detection of organophosphatase activity.
Authors:Soukharev S, Hammond DJ
Journal:Anal Biochem
PubMed ID:15033522
A new fluorogenic substrate for the specific detection of organophosphatase (OPase) activity has been designed and evaluated. Our results indicate that 7-diethylphospho-6,8-difluor-4-methylumbelliferyl (DEPFMU) is hydrolyzed specifically by the OPases, mammalian serum paraoxonase and bacterial organophosphorus hydrolase (OPH). The apparent K(m) of DEPFMU is 29 microM for OPH and 91 and ... More