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Citas y referencias (9)
Invitrogen™
EnzChek™ Elastase Assay Kit
El kit de ensayo de elastasa EnzChek proporciona un método fluorométrico sensible, cómodo y rápido para medir la actividad deMás información
| Número de catálogo | Cantidad |
|---|---|
| E12056 | 600 ensayos |
Número de catálogo E12056
Precio (USD)
858,60
Each
Cantidad:
600 ensayos
Precio (USD)
858,60
Each
El kit de ensayo de elastasa EnzChek proporciona un método fluorométrico sensible, cómodo y rápido para medir la actividad de la elastasa u otras proteasas en sistemas enzimáticos purificados, lisados de células/tejidos o para el cribado de inhibidores de detección en un formato de alto rendimiento. El sustrato del kit EnzChek es nuestro conjugado de elastina DQ etiquetado con BODIPY-FL que está altamente etiquetado para que la señal de fluorescencia se apague hasta que la digestión enzimática produzca fragmentos muy fluorescentes.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónIntensidad de la fluorescencia
Cantidad600 ensayos
Condiciones de envíoTemperatura ambiente
Propiedades de sustratoSustrato basado en proteínas
Tipo de sustratoSustrato de elastasa
Enzima dianaElastasas
Para utilizar con (aplicación)Ensayo de elastasa
Línea de productosEnzChek
Tipo de productoEnsayo de elastasa
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (9)
Citations & References
Abstract
Genetic and biochemical characterization of PrtA, an RTX-like metalloprotease from Photorhabdus.
Journal:Microbiology
PubMed ID:12777498
'Proteases play a key role in the interaction between pathogens and their hosts. The bacterial entomopathogen Photorhabdus lives in symbiosis with nematodes that invade insects. Following entry into the insect, the bacteria are released from the nematode gut into the open blood system of the insect. Here they secrete factors
Metabolic mapping of proteinase activity with emphasis on in situ zymography of gelatinases: review and protocols.
Journal:J Histochem Cytochem
PubMed ID:15150280
'Proteases are essential for protein catabolism, regulation of a wide range of biological processes, and in the pathogenesis of many diseases. Several techniques are available to localize activity of proteases in tissue sections or cell preparations. For localization of the activity of matrix metalloproteinases, in situ zymography was introduced some
Alveolar wall apoptosis causes lung destruction and emphysematous changes.
Journal:Am J Respir Cell Mol Biol
PubMed ID:12707011
'Pulmonary emphysema is characterized by alveolar wall destruction and airspace enlargement. Recent evidence indicates that epithelial or endothelial apoptosis may be involved in the pathogenesis of emphysema. Here, we describe the induction of emphysematous changes, including airspace enlargement, alveolar wall destruction, and enhanced lung distensibility, in mice receiving a single
A zinc metalloprotease inhibitor, Inh, from the insect pathogen Photorhabdus luminescens.
Journal:Microbiology
PubMed ID:12177336
The entomopathogen Photorhabdus luminescens secretes many proteins during the late stages of insect larvae infection and during in vitro laboratory culture. The authors have previously characterized and purified a 55 kDa zinc metalloprotease, PrtA, from culture supernatants of P. luminescens. PrtA is secreted via a classical type I secretory pathway
Changes in neutrophil functions in astronauts.
Journal:Brain Behav Immun
PubMed ID:15265537
Exploration class human spaceflight missions will require astronauts with robust immune systems. Innate immunity will be an essential element for the healthcare maintenance of astronauts during these lengthy expeditions. This study investigated neutrophil phagocytosis, oxidative burst, and degranulation of 25 astronauts after four space shuttle missions and in nine healthy