ELF™ 97 Endogenous Phosphatase Detection Kit

Citas y referencias (32)

Invitrogen™

ELF™ 97 Endogenous Phosphatase Detection Kit

El kit de fosfatasa endógena ELF™ 97 se puede utilizar para detectar la actividad de la fosfatasa con el sustratoMás información

Número de catálogo	Cantidad
E6601	1 kit

Número de catálogo E6601

Precio (USD)

Cantidad:

1 kit

El kit de fosfatasa endógena ELF™ 97 se puede utilizar para detectar la actividad de la fosfatasa con el sustrato de la fosfatasa ELF™ 97 que, tras la hidrólisis, produce un precipitado fluorescente amarillo-verde brillante y fotoestable en el lugar de la actividad enzimática. Este precipitado fluorescente tiene varias características espectrales únicas, incluyendo un desplazamiento Stokes extremadamente grande de 180 nm que lo hace fácilmente distinguible de la fluorescencia endógena.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Método de detecciónFluorescente

Tipo de coloranteELF 97

FormularioLíquido

Cantidad1 kit

Condiciones de envíoHielo húmedo

SustratoFosfato ELF™ 97

Propiedades de sustratoSustrato químico

Tipo de sustratoSustrato de fosfatasa

Enzima dianaFosfatasas

ColorAmarillo-verde

Emission345⁄530

Para utilizar con (aplicación)Detección de fosfatasa

Para utilizar con (equipo)Microscopio de fluorescencia

Línea de productosELF

Tipo de productoEnsayo de fosfatasa

Unit SizeEach

Contenido y almacenamiento

Almacenar en el refrigerador (2 °C a 8 °C) y proteger de la luz.

Preguntas frecuentes

Can the ELF 97 reagent be applied to live cells?

ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can ELF 97 stained cell/tissue samples be multiplexed with antibody labeling?

No. The ELF 97 reagent does not covalently attach to any cellular components and may be washed away with any subsequent antibody labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (32)

Citations & References

Abstract

Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.

Authors:Plaia TW,Josephson R,Liu Y,Zeng X,Ording C,Toumadje A,Brimble SN,Sherrer ES,Uhl EW,Freed WJ,Schulz TC,Maitra A,Rao MS,Auerbach JM

Journal:Stem cells (Dayton, Ohio)

PubMed ID:16293579

Primitive adult hematopoietic stem cells can function as osteoblast precursors.

Authors:Olmsted-Davis EA, Gugala Z, Camargo F, Gannon FH, Jackson K, Kienstra KA, Shine HD, Lindsey RW, Hirschi KK, Goodell MA, Brenner MK, Davis AR

Journal:Proc Natl Acad Sci U S A

PubMed ID:14673088

'Osteoblasts are continually recruited from stem cell pools to maintain bone. Although their immediate precursor is a plastic-adherent mesenchymal stem cell able to generate tissues other than bone, increasing evidence suggests the existence of a more primitive cell that can differentiate to both hematopoietic and mesenchymal cells. We show here ... More

Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.

Authors:Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM

Journal:Stem Cells

PubMed ID:17284651

'As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line''s characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of ... More

Osteoclast responses to lipopolysaccharide, parathyroid hormone and bisphosphonates in neonatal murine calvaria analyzed by laser scanning confocal microscopy.

Authors:Suzuki K, Takeyama S, Kikuchi T, Yamada S, Sodek J, Shinoda H

Journal:J Histochem Cytochem

PubMed ID:16087705

'Because the development and activity of osteoclasts in bone remodeling is critically dependent on cell-cell and cell-matrix interactions, we used laser confocal microscopy to study the response of osteoclasts to lipopolysaccharide (LPS; 10 microg/ml), parathyroid hormone (PTH; 10(-8) M), and bisphosphonates (BPs; 1-25 microM clodronate or 0.1-2.5 microM risedronate) in ... More

Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols.

Authors:Filgueira L

Journal:J Histochem Cytochem

PubMed ID:14966208

Osteoclasts are the only bone-resorbing cells. In addition to other specific properties, osteoclasts are characterized by their expression of tartrate-resistant acidic phosphatase (TRAP), which is usually detected using a histochemical method for light microscopy. Using ELF97 phosphatase substrate, this study describes a new fluorescence-based method for TRAP detection. The fluorescence-based ... More

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