ProcartaPlex™ Mouse Immune Monitoring Panel, 48plex

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Invitrogen™

ProcartaPlex™ Mouse Immune Monitoring Panel, 48plex

El panel 48-Plex ProcartaPlex de vigilancia inmunitaria de los ratones permite el estudio de la función inmune mediante el análisisMás información

Número de catálogo	Cantidad
EPX480-20834-901	96 pruebas

Número de catálogo EPX480-20834-901

Precio (USD)

12.603,60

Añadir al carro de la compra

Cantidad:

96 pruebas

Precio (USD)

12.603,60

Añadir al carro de la compra

El panel 48-Plex ProcartaPlex de vigilancia inmunitaria de los ratones permite el estudio de la función inmune mediante el análisis de 48 objetivos proteicos en un solo pocillo mediante la tecnología Luminex xMAP. Analice 48 objetivos como la citoquina, la quimiocina y el factor de crecimiento simultáneamente para caracterizar la respuesta inmunitaria, detectar biomarcadores y realizar la validación. El panel se suministra en un formato listo para usar con viales individuales de reactivos de captura y detección formulados a una concentración 1X, que requieren menos pipeteo y configuración experimental.

Los paneles preconfigurados ProcartaPlex se han probado exhaustivamente para determinar la capacidad combinatoria, la interferencia y la reactividad cruzada de los analitos con el fin de proporcionar el mayor nivel de validación y precisión. Todos los paneles ProcartaPlex se suministran con los reactivos necesarios para realizar el ensayo.

Lista de objetivos [región de gránulos]:
Citocinas: BAFF [67], G-CSF (CSF-3) [12], GM-CSF [42], IFN alfa [30], IFN gamma [38], IL-1 alfa [56], IL-1 beta [19], IL-2 [20], IL-3 [14], IL-4 [26], IL-5 [27], IL-6 [28], IL-7 [74], IL-9 [34], IL-10 [13], IL-12p70 [39], IL-13 [35], IL-15/IL-15R [54], IL-17A (CTLA-8) [52], IL-18 [66], IL-19 [61], IL-22 [33], IL-23 [37], IL-25 (IL-17E) [29], IL-27 [36], IL-28 [64], IL-31 [76], IL-33 [75], LIF [18], M-CSF [21], RANKL [46], TNF alfa [45]
Quimiocinas: ENA-78 (CXCL5) [57], eotaxina (CCL11) [62], GRO alfa (CXCL1) [43], IP-10 (CXCL10) [22], MCP-1 (CCL2) [51], MCP-3 (CCL7) [48], MIP-1 alfa (CCL3) [47], MIP-1 beta (CCL4) [72], MIP-2 [55], RANTES (CCL5) [44]
Factores/reguladores de crecimiento: Betacelulina (BTC) [73], leptina [65], VEGF-A [25]
Receptores solubles: IL-2R [63], IL-7R alfa [53], IL-33R (ST2) [78]

Más información sobre los ensayos ProcartaPlex para la plataforma Luminex
Los inmunoensayos ProcartaPlex están basados en los principios de un sándwich ELISA, que usa dos anticuerpos específicos unidos a diferentes epítopos de una proteína para contar todos los objetivos proteicos simultáneamente con un instrumento de Luminex. Para los ensayos ProcartaPlex multiplex se requieren tan solo 25 μl de plasma o suero, o 50 μl de sobrenadante del cultivo celular, y solo se necesitan cuatro horas para obtener los resultados analizados.

Las características incluyen:
• Resultados reproducibles y fiables: validados como panel según el estándar más alto del sector que incluye pruebas de combinación de objetivos de proteínas y reactividad cruzada
• Más resultados por muestra: miden múltiples objetivos de proteínas simultáneamente en una única muestra de 25–50 μl
• Plataforma de multiplexación de tecnología Luminex bien establecida de alta referencia para la detección y cuantificación de proteínas

Los ensayos de ProcartaPlex utilizan la tecnología Luminex xMAP (perfil multianalito) para la detección y cuantificación simultáneas de hasta 65 objetivos proteicos en una sola muestra de plasma, suero, sobrenadantes de cultivo celular y otros fluidos corporales de 25–50 μl.

Las microesferas de Luminex del ensayo de ProcartaPlex se tiñen internamente con proporciones precisas de fluoróforos rojos e infrarrojos para crear firmas específicas que pueden ser identificadas por los sistemas de detección Luminex xMAP (por ejemplo, Luminex 200, FLEXMAP 3D y MAGPIX). El ensayo ProcartaPlex, similar a un ELISA tipo sándwich, utiliza pares de anticuerpos emparejados para identificar la proteína de interés. En un ensayo multiplex, cada gránulo espectralmente único se etiqueta con anticuerpos específicos para una sola proteína diana, y las proteínas vinculadas se identifican con anticuerpos biotinilados y estreptavidina–R-ficoeritrina (RPE). La conjugación de anticuerpos específicos de proteínas en un gránulo distinto permite el análisis de varios elementos diana en un solo pocillo.

La diferencia más significativa entre un ensayo ProcartaPlex y uno ELISA es que

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Intervalo del ensayoVer certificado de análisis

Sensibilidad del ensayoSee Certificate of Analysis

FormatoKit multiplex

CV interensayoMenos del 15 %

CV intraensayoMenos del 15 %

Línea de productosProcartaPlex

Tipo de muestraSobrenadantes de cultivo celular, suero y plasma, Plasma, Cell Culture Supernatants

Volumen de muestraSuero, plasma: 25 μl; CCS: 50 μl

Condiciones de envíoHielo húmedo

CombinabilityNo combinable

Tipo de productoPanel de vigilancia inmunológica

Cantidad96 pruebas

Research AreaImmunology

EspecieRatón

Unit SizeEach

Contenido y almacenamiento

• 2 vials Mouse Standard Mix D (lyophilized)
• 1 vial Capture Bead Mix (1X)
• 1 vial Biotinylated Detection Antibody Mix (1X)
• 2 vials Mouse Control-High
• 2 vials Mouse Control-Low
• 1 Bottle Reading Buffer (1X)
• 1 Bottle Wash Buffer (10X)
• 1 Bottle Streptavidin-PE (1X)
• 1 Bottle Universal Assay Buffer (1X)
• 8-tube strip
• Adhesive film
• Flat-bottom 96-well plate, black

Store (at 2°C to 8°C.)

Preguntas frecuentes

What is the size of the Luminex beads you currently use?

The beads used in our Luminex instrument-compatible ProcartaPlex and QuantiGene Plex assays are 6.5 micron superparamagnetic beads.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am interested in performing Luminex assays using BioSource kits, and I have a Luminex xMAP system. Besides the kits and system, what other reagents and equipment will I need?

The following is a list of general lab supplies that are required for running BioSource immunoassays on the Luminex xMAP system:
1) Sonicating water bath
2) Orbital shaker
3) Vortexer
4) Repeating and/or multi-channel pipetter (not required, but recommended)
5) Calibrated adjustable precision pipettes, with disposable plastic tips
6) Glass/plastic tubes and racks for preparing reagents
7) Graduated cylinder and container for preparing wash solution
8) Aluminum foil
9) Deionized or distilled water.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the Luminex beads require special care in handling?

The Luminex beads should be protected from light because they are susceptible to photobleaching. We recommend protecting the beads by keeping containers covered with aluminum foil during all incubation steps, and exercising care during handling. The beads should not be frozen, subjected to excessive heat, or exposed to organic solvents.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why would the Luminex acquisition software display "Sample Empty" messages during analysis?

(1) The user did not properly aliquot the diluted beads, such that no beads were actually added to the wells (make sure that the bead concentrates are sonicated and vortexed well, then check the pipet tip to ensure that air bubbles were not drawn up)
(2) The user missed loading diluted beads to some wells, which is likely since the small volume is clear and difficult to visualize in the clear plastic plate (we have now addressed this customer difficulty by coloring each of the Buffer Reagent Kit components)
(3) The user applied too much vacuum pressure at some point during the wash steps, or allowed the pressure to spike even once, such that the filter membrane tore in a few wells releasing the beads (make sure that the vacuum manifold pressure is kept below 5mm/in Hg, depending on their system -- a good rule of thumb is that it should take a full 3-second count to GENTLY empty the wells of 200uL)
(4) The user did not properly sonicate and vortex the beads prior to dilution, such that the percent of bead aggregation was high and the instrument was unable to find enough single beads to meet the events/bead value designated by the customer (make sure that the Bead Concentrate tube is put into the waterbath all the way to the cap, since the tube is hollow until the top third)
(5) The user lost beads by shaking the plate too aggressively or handling it improperly (make sure that the orbital shaker is set to a speed that allows for maximum vortex in the wells without spillage)
(6) The user exposed the beads to an excess of light during storage or running of the assay, such that some but not all of the beads were photobleached and therefore falling outside the acceptable range for each bead region (make sure that the plate is covered on the top/sides with foil throughout the assay, away from Windows and spotlights, and that the bead component of the kits is stored in the dark)*
(7) There was a clog in the sample needle, such that the instrument was unable to take up enough sample to meet the number of events requested per bead region (suggest that the user follow the manual instructions for dislodging a clog, which include several Back Flush steps and may require removal of the needle for sonication with probe alignment).

* Some of the older Antibody Bead Kits still have clear plastic tops instead of black ones. In cases where customers store kits in lit refrigerators, or keep them open on the lab bench, even a few hours of light exposure is enough to photobleach beads. It is important to note, in general, that higher number bead regions are more susceptible to photobleaching. In order to draw conclusions about the source of the difficulty, we would ask to see the data, specifically the Masterplex QT file, which would enable us to examine the pattern of "Sample Empty" occurrences in addition to the bead counts per well.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the Luminex beads made of?

The beads are made of polystyrene.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all ProcartaPlex™ Mouse Immune Monitoring Panel, 48plex FAQs

Citations & References (1)

Citations & References

Abstract

Fentanyl Overdose Causes Prolonged Cardiopulmonary Dysregulation in Male SKH1 Mice.

Authors:Newman M,Connery H,Kannan S,Gautam A,Hammamieh R,Chakraborty N,Boyd J

Journal:Pharmaceuticals (Basel, Switzerland)

PubMed ID:39065791

Fentanyl overdose is a survivable condition that commonly resolves without chronic overt changes in phenotype. While the acute physiological effects of fentanyl overdose, such as opioid-induced respiratory depression (OIRD) and Wooden Chest Syndrome, represent immediate risks of lethality, little is known about longer-term systemic or organ-level impacts for survivors. In ... More