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ProcartaPlex™ Human Immune Monitoring Panel for MAGPIX, 65plex
ProcartaPlex™ Human Immune Monitoring Panel for MAGPIX, 65plex
Citas y referencias (1)
Invitrogen™

ProcartaPlex™ Human Immune Monitoring Panel for MAGPIX, 65plex

El panel ProcartaPlex 65-plex de supervisión humana inmune para MAGPIX es un kit de inmunoensayo multiplex preconfigurado que mide 65Más información
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Número de catálogoCantidad
EPX650-16500-90196 pruebas
Número de catálogo EPX650-16500-901
Precio (USD)
-
Cantidad:
96 pruebas
El panel ProcartaPlex 65-plex de supervisión humana inmune para MAGPIX es un kit de inmunoensayo multiplex preconfigurado que mide 65 citocinas, quimiocinas y factores de crecimiento mediante la tecnología Luminex Xmap. Analice estos 65 objetivos simultáneamente para obtener un perfil eficaz de la respuesta inmunitaria, descubrimiento de biomarcadores y validación. Este kit consta de dos kits independientes: Un kit de 43 y un kit de 22 plex que se han diseñado específicamente para usarse en el instrumento MAGPIX que es capaz de detectar hasta 50 objetivos simultáneamente. Los usuarios de Luminex 100 y FLEXMAP 3D deben utilizar el panel de inmunomonitorización 65 plex (Cat. EPX650-10065-901) que contiene los 65 objetivos en un solo panel. Los paneles (43-plex y 22-plex) se suministran en un formato listo para usar con viales individuales de reactivos de captura y detección formulados a una concentración 1X, que requieren menos pipeteo y configuración experimental.

Los paneles preconfigurados ProcartaPlex se han probado exhaustivamente para detectar la combinabilidad, interferencia y reactividad cruzada de los analitos con el fin de proporcionar el mayor nivel de validación y precisión. Todos los paneles ProcartaPlex se suministran con los reactivos necesarios para realizar el ensayo.

Los paneles multiplex ProcartaPlex están disponibles en múltiples formatos en seis especies (humano, ratón, rata, primate no humano, porcino y canino).

Lista de dianas [región de gránulos]:
22-plex: APRIL [52], BAFF [65], BLC (CXCL13) [15], CD30 [34], ENA-78 (CXCL5) [55], Eotaxin-2 (CCL24) [37], Eotaxin-3 (CCL26) [77], FGF-2 [75], Fractalkine (CX3CL1) [39], IL-16 [47], IL-2R (CD25) [22], IL-20 [26], I-TAC (CXCL11) [46], MCP-2 (CCL8) [18], MCP-3 (CCL7) [66], MDC (CCL22) [30], MIF [67], MIG (CXCL9) [54], TNF-RII [45], TRAIL (CD253) [29], TSLP [61], TWEAK [35]
43-plex: CD40L (CD154) [74], eotaxina (CCL11) [33], Gro-alfa (CXCL1) [61], G-CSF (CSF-3) [42], GM-CSF [44], HGF [46], IFN alfa [48], IP-10 (CXCL10) [22], IFN gamma [43], IL-1 alfa [62], IL-1 beta [18], IL-2 [19], IL-3 [73], IL-4 [20], IL-5 [21], IL-6 [25], IL-7 [26], IL-8 (CXCL8) [27], IL-9 [52], IL-10 [28], IL-12p70 [34], IL-13 [35], IL-15 [65], IL-17A (CTLA-8) [36], IL-18 [66], IL-21 [72], IL-22 [76], IL-23 [63], IL-27 [14], IL-31 [37], LIF [15], M-CSF [67], MCP-1 (CCL2) [51], MIP-1 alfa (CCL3) [12], MIP-1 beta (CCL4) [47], MIP-3 alfa (CCL20) [56], MMP-1 [64], NGF beta [55], SDF-1 alfa (CXCL12) [13], SCF [39], TNF alfa [45], TNF beta [54], VEGF-A [78]

Acerca de los ensayos ProcartaPlex para la plataforma Luminex
Los inmunoensayos ProcartaPlex están basados en los principios de un ELISA tipo sándwich. Utilizan dos anticuerpos específicos que se enlazan a diferentes epítopos de una proteína para cuantificar todas las proteínas diana de forma simultánea, mediante el uso de un instrumento de Luminex. Los ensayos multiplex ProcartaPlex requieren tan solo 25 µl de plasma o suero, o 50 µl de sobrenadante de cultivo celular, y solo cuatro horas para obtener los resultados analizados.

Las características incluyen:
• Resultados reproducibles y fiables: validados como panel según el estándar más alto del sector que incluye pruebas de combinación de objetivos de proteínas y reactividad cruzada
• Más resultados por muestra: miden múltiples objetivos de proteínas simultáneamente en una única muestra de 25–50 µl
•Plataforma de multiplexación de tecnología Luminex bien establecida de alta referencia para la detección y cuantificación de proteínas

Los ensayos de ProcartaPlex utilizan la tecnología Luminex xMAP (perfil multianalito) para la detección y cuantificación simultáneas de hasta 65 objetivos proteicos en una sola muestra de plasma, suero, sobrenadantes de cultivo celular y otros fluidos corporales de 25–50 µl.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Intervalo del ensayoVer certificado de análisis
Sensibilidad del ensayoMenos del 15 %
Método de detecciónFluorescente
Para utilizar con (equipo)Instrumentos Luminex™
FormatoKit multiplex
Línea de productosProcartaPlex
Tipo de muestraSobrenadantes de cultivo celular, suero y plasma, Plasma, Cell Culture Supernatants
Volumen de muestraSuero, plasma: 25 μl; CCS: 50 μl
Condiciones de envíoHielo húmedo
CombinabilityNo combinable
Tipo de productoPanel de supervisión inmunológica para MAGPIX
Cantidad96 pruebas
Research AreaImmunology
EspecieHumano
Unit SizeEach
Contenido y almacenamiento
  • 4 viales de mezcla estándar humana M (liofilizada)
  • 1 vial de mezcla de gránulos de captura M1 (1X)
  • 1 vial de mezcla de gránulos de captura M2 (1X)
  • 1 vial de mezcla de anticuerpos de detección biotinilada M1 (1X)
  • 1 vial de mezcla de anticuerpos de detección biotinilada M2 (1X)
  • 4 viales de control alto humano alto
  • 4 viales de control alto humano bajo
  • 1 frasco de tampón de lectura (1X)
  • 1 frasco de tampón de lavado (10X)
  • 1 frasco de estreptavidina-PE (1X)
  • 1 frasco de tampón de ensayo universal (1X)
  • Tira de 8 tubos
  • Película adhesiva
  • 2 placas de 96 pocillos de fondo plano, negras
  • Almacenar entre 2 °C y 8 °C

Preguntas frecuentes

What is the size of the Luminex beads you currently use?

The beads used in our Luminex instrument-compatible ProcartaPlex and QuantiGene Plex assays are 6.5 micron superparamagnetic beads.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am interested in performing Luminex assays using BioSource kits, and I have a Luminex xMAP system. Besides the kits and system, what other reagents and equipment will I need?

The following is a list of general lab supplies that are required for running BioSource immunoassays on the Luminex xMAP system:
1) Sonicating water bath
2) Orbital shaker
3) Vortexer
4) Repeating and/or multi-channel pipetter (not required, but recommended)
5) Calibrated adjustable precision pipettes, with disposable plastic tips
6) Glass/plastic tubes and racks for preparing reagents
7) Graduated cylinder and container for preparing wash solution
8) Aluminum foil
9) Deionized or distilled water.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the Luminex beads require special care in handling?

The Luminex beads should be protected from light because they are susceptible to photobleaching. We recommend protecting the beads by keeping containers covered with aluminum foil during all incubation steps, and exercising care during handling. The beads should not be frozen, subjected to excessive heat, or exposed to organic solvents.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why would the Luminex acquisition software display "Sample Empty" messages during analysis?

(1) The user did not properly aliquot the diluted beads, such that no beads were actually added to the wells (make sure that the bead concentrates are sonicated and vortexed well, then check the pipet tip to ensure that air bubbles were not drawn up)
(2) The user missed loading diluted beads to some wells, which is likely since the small volume is clear and difficult to visualize in the clear plastic plate (we have now addressed this customer difficulty by coloring each of the Buffer Reagent Kit components)
(3) The user applied too much vacuum pressure at some point during the wash steps, or allowed the pressure to spike even once, such that the filter membrane tore in a few wells releasing the beads (make sure that the vacuum manifold pressure is kept below 5mm/in Hg, depending on their system -- a good rule of thumb is that it should take a full 3-second count to GENTLY empty the wells of 200uL)
(4) The user did not properly sonicate and vortex the beads prior to dilution, such that the percent of bead aggregation was high and the instrument was unable to find enough single beads to meet the events/bead value designated by the customer (make sure that the Bead Concentrate tube is put into the waterbath all the way to the cap, since the tube is hollow until the top third)
(5) The user lost beads by shaking the plate too aggressively or handling it improperly (make sure that the orbital shaker is set to a speed that allows for maximum vortex in the wells without spillage)
(6) The user exposed the beads to an excess of light during storage or running of the assay, such that some but not all of the beads were photobleached and therefore falling outside the acceptable range for each bead region (make sure that the plate is covered on the top/sides with foil throughout the assay, away from Windows and spotlights, and that the bead component of the kits is stored in the dark)*
(7) There was a clog in the sample needle, such that the instrument was unable to take up enough sample to meet the number of events requested per bead region (suggest that the user follow the manual instructions for dislodging a clog, which include several Back Flush steps and may require removal of the needle for sonication with probe alignment).

* Some of the older Antibody Bead Kits still have clear plastic tops instead of black ones. In cases where customers store kits in lit refrigerators, or keep them open on the lab bench, even a few hours of light exposure is enough to photobleach beads. It is important to note, in general, that higher number bead regions are more susceptible to photobleaching. In order to draw conclusions about the source of the difficulty, we would ask to see the data, specifically the Masterplex QT file, which would enable us to examine the pattern of "Sample Empty" occurrences in addition to the bead counts per well.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the Luminex beads made of?

The beads are made of polystyrene.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all ProcartaPlex™ Human Immune Monitoring Panel for MAGPIX, 65plex FAQs

Citations & References (1)

Citations & References
Abstract
Multiplexing protein and gene level measurements on a single Luminex platform.
Authors:Cook DB, McLucas BC, Montoya LA, Brotski CM, Das S, Miholits M, Sebata TH
Journal:Methods
PubMed ID:30742996
'The ability to simultaneously measure multiple secreted proteins and the corresponding gene expression levels from a single sample is valuable for comprehensive analysis. Bottlenecks to traditional immunoassays and gene expression assays include large sample consumption, time consuming experimental procedures, and complex data analysis.' ... More