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Citas y referencias (99)
Invitrogen™
FluoReporter™ lacZ Flow Cytometry Kit
Kit de citometría de flujo FluoReporter™ lacZ
| Número de catálogo | Cantidad |
|---|---|
| F1930 también denominado F-1930 | 1 kit |
Número de catálogo F1930
también denominado F-1930
Precio (USD)
-
Cantidad:
1 kit
The FluoReporter™ lacZ Flow Cytometry Kit provides materials and protocols for quantitating β-galactosidase activity with fluorescein di-V-galactoside (FDG) in single cells using flow cytometry. This kit is accompanied by a license to practice patented techniques for loading FDG by hypotonic shock and improving retention of fluorescein in lacZ-positive cells.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
FormularioSolución
Línea de productosFluoReporter
Tipo de productoSistema de ensayo de gen indicador de β-galactosidasa
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Unit SizeEach
Contenido y almacenamiento
Contiene 1 vial de FDG (0,5 ml, 20 mm en agua/DMSO/etanol, 8:1:1, v/v), 1 vial de PETG (2,0 ml, 50 mm en agua), difosfatos de cloroquina (1,0 ml, 30 mm en agua) y 1 vial de yoduro de propidio (1,0 ml, 150 µm en agua).
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Citations & References (99)
Citations & References
Abstract
Detection of rare antigen-presenting cells by the lacZ T-cell activation assay suggests an expression cloning strategy for T-cell antigens.
Journal:Proc Natl Acad Sci U S A
PubMed ID:1378619
'The alpha/beta T-cell receptor a complex ligand formed by the association of antigenic peptides with molecules of the major histocompatibility complex (MHC). The inherent limitations of the conventional T-cell activation assays used to detect these peptide/MHC ligands have, until now, hampered the development of expression cloning systems for T-cell antigens.
Primitive adult hematopoietic stem cells can function as osteoblast precursors.
Journal:Proc Natl Acad Sci U S A
PubMed ID:14673088
'Osteoblasts are continually recruited from stem cell pools to maintain bone. Although their immediate precursor is a plastic-adherent mesenchymal stem cell able to generate tissues other than bone, increasing evidence suggests the existence of a more primitive cell that can differentiate to both hematopoietic and mesenchymal cells. We show here
Retroviral gene transfer is inhibited by chondroitin sulfate proteoglycans/glycosaminoglycans in malignant pleural effusions.
Journal:J Biol Chem
PubMed ID:9115227
'Gene therapy may be an important adjuvant for treating cancer in the pleural space. The initial results of retroviral gene transfer to cancer cells in malignant pleural effusions revealed that transduction was markedly inhibited, and studies to characterize the inhibitory factor(s) were performed. The inhibition was contained within the soluble,
In situ detection of transcriptionally active chromatin and genetic regulatory elements in individual viable mammalian cells.
Journal:Immunol Suppl
PubMed ID:2807403
'Using a newly developed FACS method for quantifying the expression of the Escherischia coli lacZ reporter gene in viable mammalian cells, we have obtained cloned cell lines in which the expression of lacZ is under the control of native endogenous transcription elements. We infected the murine pre-B cell 70Z/3 with
Transcriptional activity of the neuron-specific enolase (NSE) promoter in murine embryonic stem (ES) cells and preimplantation embryos.
Journal:Eur J Cell Biol
PubMed ID:7925488
'Mouse embryonic stem (ES) cells were transfected with a plasmid composed of an E. coli lacZ gene fused to 1.8 kb of rat neuron-specific enolase (NSE) promoter sequences. While this reporter construct had been shown previously to function exclusively in postmitotic neurons and neuro-endocrine cells of transgenic mice, stably transfected