Fc OxyBURST™ Green Assay Reagent
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Citas y referencias (14)
Invitrogen™

Fc OxyBURST™ Green Assay Reagent

El reactivo de ensayo Fc OxyBURST™ Green se compone de seroalbúmina bovina (BSA) que se ha vinculado covalentemente a laMás información
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Número de catálogoCantidad
F290225 reacciones
Número de catálogo F2902
Precio (USD)
-
Cantidad:
25 reacciones
El reactivo de ensayo Fc OxyBURST™ Green se compone de seroalbúmina bovina (BSA) que se ha vinculado covalentemente a la diclorodifluoresceína (H2DCF) y, a continuación, se ha complexado con un anticuerpo policlonal de conejo antiBSA purificado. Cuando este inmunocomplejo se une a los receptores Fc, las moléculas no fluorescentes H2DCF se internalizan dentro del fagovacuoloe y posteriormente se oxidan para formar 2''7'-diclorofluoresceína verde fluorescente.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración3 mg⁄ml
Método de detecciónFluorescente
Tipo de coloranteSensor de radicales libres
FormularioSolución
FormatoTubos, portaobjetos
Cantidad25 reacciones
Condiciones de envíoHielo húmedo
SolubilidadDMSO (dimetilsulfóxido)
Emission488⁄523
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo
Línea de productosOXYBURST
Tipo de productoReactivo de ensayo
Unit SizeEach
Contenido y almacenamiento
Contiene un vial de reactivo de ensayo verde Fc OxyBURST™ (suspensión en PBS que contiene azida sódica). Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Citations & References (14)

Citations & References
Abstract
The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcgammaIIA receptor-induced phagocytosis.
Authors:Suh CI, Stull ND, Li XJ, Tian W, Price MO, Grinstein S, Yaffe MB, Atkinson S, Dinauer MC
Journal:J Exp Med
PubMed ID:16880255
'Superoxide produced by the phagocyte reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is essential for host defense. Enzyme activation requires translocation of p67(phox), p47(phox), and Rac-GTP to flavocytochrome b558 in phagocyte membranes. To examine the regulation of phagocytosis-induced superoxide production, flavocytochrome b558, p47(phox), p67(phox), and the FcgammaIIA receptor were expressed ... More
Severe impairment in early host defense against Listeria monocytogenes in mice deficient in acid sphingomyelinase.
Authors:Utermöhlen O, Karow U, Löhler J, Krönke M
Journal:J Immunol
PubMed ID:12594290
'The phagolysosomal compartment is crucial for the defense against infection with intracellular pathogens. Within this compartment, the TNF- and IFN-gamma-responsive acid sphingomyelinase (ASMase) generates the signaling molecule ceramide, resulting in the activation of proteases like cathepsin D. To investigate the possible role of ASMase as a mediator of the antibacterial ... More
Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate (H2DCF-DA) or dihydrorhodamine 123 (H2R123).
Authors:Trayner ID, Rayner AP, Freeman GE, Farzaneh F
Journal:J Immunol Methods
PubMed ID:7594627
'It is well established that the fluorescent probes dichlorodihydrofluorescein diacetate (H2DCF-DA) and dihydrorhodamine 123 (H2R123) can be used to detect the respiratory burst response of mature myeloid cells. We describe a simple, fast and quantitative assay for myeloid differentiation based on the oxidation of these probes, which can be performed ... More
A cytosolic calcium transient is not necessary for degranulation or oxidative burst in immune complex-stimulated neutrophils.
Authors:Seetoo KF, Schonhorn JE, Gewirtz AT, Zhou MJ, McMenamin ME, Delva L, Simons ER
Journal:J Leukoc Biol
PubMed ID:9307071
'Receptor-mediated activation of neutrophils (PMN) initiates possibly interdependent events, including a rapid transient increase in [Ca2+]i, implicated as a second messenger. To investigate whether this transient is required for eventual degranulation, PMN were incubated with an intracellular Ca2+ chelator (BAPTA), then exposed to chemotactic peptide [N-formyl-methionyl-leucyl-phenylalanine (fMLP)l with or without ... More
Fluorescent probes for living cells.
Authors:Johnson I
Journal:Histochem J
PubMed ID:10188922
'The functional characteristics of fluorescent probes used for imaging and measuring dynamic processes in living cells are reviewed. Initial consideration is given to general design requirements for delivery, targeting, detectability and fluorescence readout, and current technologies for attaining them. Discussion then proceeds to the more application-specific properties of intracellular ion ... More
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