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Citas y referencias (14)
Invitrogen™
FluoReporter™ Blue Fluorometric dsDNA Quantitation Kit, 200-2,000 assays
El kit de cuantificación de ADNbc fluorimétrico azul FluoReporter™ proporciona los protocolos y reactivos para el NA de análisis celularMás información
| Número de catálogo | Cantidad |
|---|---|
| F2962 | De 200 a 2000 ensayos |
Número de catálogo F2962
Precio (USD)
-
Cantidad:
De 200 a 2000 ensayos
El kit de cuantificación de ADNbc fluorimétrico azul FluoReporter™ proporciona los protocolos y reactivos para el NA de análisis celular con la tinción de ácido nucleico Hoechst 33258 azul fluorescente. Con este kit, la cuantificación del ADN celular es rápida, y todas las manipulaciones se pueden realizar en pocillos de microplaca Las células se lisan congelándolas en agua destilada, se añade después una solución de colorante diluido a las células lisadas y se mide la fluorescencia
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (equipo)Lector de microplacas
Línea de productosFluoReporter
Tipo de productoKit de cuantificación de ADNbc
CantidadDe 200 a 2000 ensayos
Condiciones de envíoTemperatura ambiente
Método de detecciónFluorescencia
FormatoPlaca de 96 pocillos
Etiqueta o tinteOtras etiquetas o colorantes
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8 °C) y proteger de la luz.
Preguntas frecuentes
Can I use Hoechst dyes to do both DNA quantitation and cell number counting in plates?
Citations & References (14)
Citations & References
Abstract
Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytes.
Journal:Proc Natl Acad Sci U S A
PubMed ID:14688407
'Hormonal induction of growth-arrested 3T3-L1 preadipocytes rapidly activates expression of CCAAT/enhancer-binding protein (C/EBP) beta. Acquisition of DNA-binding activity by C/EBPbeta, however, is delayed until the cells synchronously enter the S phase of mitotic clonal expansion (MCE). After MCE, C/EBPbeta activates expression of C/EBPalpha and peroxisome proliferator-activated receptor gamma, which then
In vivo imaging platform for tracking immunotherapeutic cells.
Journal:Nat Biotechnol
PubMed ID:16041364
Cellular therapeutics show great promise for the treatment of disease, but few noninvasive techniques exist for monitoring the cells after administration. Here we present a magnetic resonance imaging (MRI) technology that uses perfluoropolyether (PFPE) agents to track cells in vivo. Fluorine MRI selectively images only the labeled cells, and a
ATP regulates the differentiation of mammalian skeletal muscle by activation of a P2X5 receptor on satellite cells.
Journal:J Cell Biol
PubMed ID:12135987
ATP is well known for its role as an intracellular energy source. However, there is increasing awareness of its role as an extracellular messenger molecule (Burnstock, 1997). Although evidence for the presence of receptors for extracellular ATP on skeletal myoblasts was first published in 1983 (Kolb and Wakelam), their physiological
5-HT2A receptor induces ERK phosphorylation and proliferation through ADAM-17 tumor necrosis factor-alpha-converting enzyme (TACE) activation and heparin-bound epidermal growth factor-like growth factor (HB-EGF) shedding in mesangial cells.
Journal:J Biol Chem
PubMed ID:16737974
In this study, we present multiple lines of evidence to support a critical role for heparin-bound EGF (epidermal growth factor)-like growth factor (HB-EGF) and tumor necrosis factor-alpha-converting enzyme (TACE) (ADAM17) in the transactivation of EGF receptor (EGFR), ERK phosphorylation, and cellular proliferation induced by the 5-HT(2A) receptor in renal mesangial
Small proline-rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts.
Journal:FASEB J
PubMed ID:32297675