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Thermo Scientific™
Kit de Panel 1.1 de genotipos caninos
Thermo Scientific Canine Genotypes Panel 1.1 is a reagents kit for canine parentage testings and identification using Short Tandem RepeatMás información
| Número de catálogo | Quantity |
|---|---|
| F860S |
Número de catálogo F860S
Precio (USD)
-
Thermo Scientific Canine Genotypes Panel 1.1 is a reagents kit for canine parentage testings and identification using Short Tandem Repeat (STR) DNA loci. Due to their high level of informativeness and Mendelian inheritance, microsatellites have become the markers of choice for parentage testing, identification and forensics.
The Canine Genotypes Panel 1.1 provides all the necessary reagents for amplification of the 19 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Canine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Canine Genotypes Panel 1.1 kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'. One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument.
Canine Genotypes Panel 1.1 encompasses the following 19 loci: AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHTh171, REN247M23 and Amelogenin (sex determining locus). These markers are included in the 'core panel' of loci recommended by the Applied Genetics Committee of Companying Animals of the International Society for Animal Genetics (ISAG).
Highlights
• Single multiplex PCR of 19 STR loci
• Master mix and Primer mix provided for easy pipetting
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calls represents true alleles (no plus-A peaks)
The Canine Genotypes Panel 1.1 provides all the necessary reagents for amplification of the 19 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Canine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Canine Genotypes Panel 1.1 kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'. One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument.
Canine Genotypes Panel 1.1 encompasses the following 19 loci: AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHTh171, REN247M23 and Amelogenin (sex determining locus). These markers are included in the 'core panel' of loci recommended by the Applied Genetics Committee of Companying Animals of the International Society for Animal Genetics (ISAG).
Highlights
• Single multiplex PCR of 19 STR loci
• Master mix and Primer mix provided for easy pipetting
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calls represents true alleles (no plus-A peaks)
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónElectroforesis capilar
Para utilizar con (aplicación)PCR
N.º de reacciones100 reacciones
Tipo de productoKit de Panel 1.1 de genotipos caninos
ObjetivoPruebas de genotipado y parentesco canino
TécnicaAnálisis de STR (repetición en tándem corto)
Unit SizeEach
Contenido y almacenamiento
Contiene:
• 1,1 ml de mezcla maestra de Panel 1.1 de genotipos caninos
• 1,1 ml de mezcla de cebadores de Panel 1.1 de genotipos caninos
• 30 µl de control de genotipos caninos ADN001
Almacenar a -20 °C. La mezcla de cebadores debe protegerse de la luz en todo momento.
• 1,1 ml de mezcla maestra de Panel 1.1 de genotipos caninos
• 1,1 ml de mezcla de cebadores de Panel 1.1 de genotipos caninos
• 30 µl de control de genotipos caninos ADN001
Almacenar a -20 °C. La mezcla de cebadores debe protegerse de la luz en todo momento.