FluoSpheres™ Carboxylate-Modified Microspheres
FluoSpheres™ Carboxylate-Modified Microspheres
Citas y referencias (4)
Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Consiga la fluorescencia más brillante gracias a las microesferas FluoSphere modificadas con carboxilos, disponibles en diferentes colores y tamaños de partículas.
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Número de catálogoDiámetro (métrico)ColorExcitación/emisiónCantidad
F8800
también denominado F-8800
0,1 μmNaranja540/560 nm10 mL
F88030,1 μmAmarillo-verde505/515 nm10 mL
F88161,0 μmCarmesí625/645 nm2 mL
F88231,0 μmAmarillo-verde505/515 nm10 mL
F88010,1 μmRojo580/605 nm10 mL
F88110,2 μmAmarillo-verde505/515 nm10 mL
F88070,2 μmRojo oscuro660/680 nm2 mL
F107200,04 μmAmarillo-verde, Naranja, Rojo, Rojo oscuro505/515, 540/560, 580/605, 660/680 nm1 ml (cada uno)
F208810,2 μmNaranja365/610 nm2 mL
F8783
también denominado F-8783
0,02 μmRojo oscuro660/680 nm2 mL
F87860,02 μmRojo580/605 nm10 mL
F8795
también denominado F-8795
0,04 μmAmarillo-Verde505/515 nm1 mL
F88130,5 μmAmarillo-verde505/515 nm10 mL
F88201,0 μmNaranja540/560 nm10 mL
F88252,0 μmRojo Nilo535/575 nm2 mL
F88272,0 μmAmarillo-verde505/515 nm2 mL
F8781
también denominado F-8781
0,02 μmAzul365/415 nm10 mL
F8782
también denominado F-8782
0,02 μmCarmesí625/645 nm2 mL
F87840,02 μmRojo Nilo535/575 nm10 mL
F87870,02 μmAmarillo-verde505/515 nm10 mL
F8789
también denominado F-8789
0,04 μmRojo oscuro660/680 nm1 mL
F8792
también denominado F-8792
0,04 μmNaranja540/560 nm1 mL
F87930,04 μmRojo580/605 nm1 mL
F87940,04 μmRojo-naranja565/580 nm1 mL
F87970,1 μmAzul350/440 nm10 mL
F87990,1 μmInfrarrojo715/755 nm1 mL
F88050,2 μmAzul365/415 nm10 mL
F88060,2 μmCarmesí625/645 nm2 mL
F88090,2 μmNaranja540/560 nm10 mL
F88100,2 μmRojo580/605 nm10 mL
F88120,5 μmRojo580/605 nm10 mL
F88141,0 μmAzul365/415 nm10 mL
F88151,0 μmAzul350/440 nm10 mL
F88191,0 μmRojo Nilo535/575 nm10 mL
F88211,0 μmRojo580/605 nm10 mL
F8824
también denominado F-8824
2,0 μmAzul365/415 nm2 mL
F88262,0 μmRojo580/605 nm2 mL
Número de catálogo F8800
también denominado F-8800
Precio (USD)
-
Diámetro (métrico):
0,1 μm
Color:
Naranja
Excitación/emisión:
540/560 nm
Cantidad:
10 mL

Utilice nuestra amplia selección de microesferas modificadas con carboxilatos FluoSpheres para realizar fácilmente citometría de flujo, microscopía, HTS, HCS, inmunoensayo, así como otras aplicaciones de laboratorio. Los gránulos FluoSphere se pueden utilizar en adsorción pasiva o activa, acoplamiento covalente de proteínas, ácidos nucleicos y biomoléculas para aplicaciones de captura de partículas. Las microesferas FluoSphere están cargadas de colorantes fluorescentes exclusivos, lo que las convierte en las microesferas más brillantes disponibles.

Visualice la fluorescencia más brillante para aplicaciones de laboratorio, como microscopía de fluorescencia, citometría de flujo, HTS, HCS y rastreo celular, gracias a nuestras microesferas FluoSphere modificadas con carboxilatos, fabricadas con microesferas de poliestireno y cargadas con diferentes colorantes patentados. Gracias al uso de métodos de coloración especializados, permite que todas las moléculas de colorante fluorescente estén contenidas dentro de cada microesfera de poliestireno, en lugar de en la superficie. Este entorno de protección dentro del gránulo protege el tinte de efectos medioambientales perjudiciales, como la decoloración fotográfica. Nuestras microesferas modificadas con carboxilato están recubiertas con un polímero hidrofílico que contiene múltiples ácidos carboxílicos para la fijación covalente de los ligandos. Existen diversos tamaños de partículas disponibles para diferentes usos y experimentos de investigación.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Excitación/emisión540/560 nm
Línea de productosFLUOSPHERES
Cantidad10 mL
Modificación de la superficieCarboxilato
ColorNaranja
Diámetro (métrico)0,1 μm
Para utilizar con (aplicación)Microscopía de fluorescencia
MaterialPoliestireno
Tipo de productoMicroesfera modificada con carboxilatos
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

Citations & References (4)

Citations & References
Abstract
Measurement of monomer-oligomer distributions via fluorescence moment image analysis.
Authors:Sergeev M, Costantino S, Wiseman PW
Journal:Biophys J
PubMed ID:16935950
We present higher-order moment analysis of fluorescence intensity fluctuations from individual laser scanning microscopy images applied to study monomer-oligomer distributions. We demonstrate that the number densities and brightness ratios of a mixed population of monomers and oligomers can be determined by analyzing higher-order moments of the fluorescence intensity fluctuations from ... More
Analysis of several fluorescent detector molecules for protein microarray use.
Authors:Wiese R
Journal:Luminescence
PubMed ID:12536376
The utility of several streptavidin-linked fluorescent detector molecules was evaluated on two protein microarray platforms. Tested detector molecules included: Alexa Fluor 546; R-phycoerythrin (RPE), orange fluospheres; Cy3-containing liposomes (Large Unilamellar Vesicles, LUV) labelled with Cy3; and an RPE-antibody complex. The two array architectures tested consisted of an array of murine ... More
In situ fluorescent protein imaging with metal film-enhanced total internal reflection microscopy.
Authors:Burghardt TP, Charlesworth JE, Halstead MF, Tarara JE, Ajtai K
Journal:Biophys J
PubMed ID:16565065
Fluorescence detection of single molecules provides a means to investigate protein dynamics minus ambiguities introduced by ensemble averages of unsynchronized protein movement or of protein movement mimicking a local symmetry. For proteins in a biological assembly, taking advantage of the single molecule approach could require single protein isolation from within ... More
In situ single-molecule imaging with attoliter detection using objective total internal reflection confocal microscopy.
Authors:Burghardt TP, Ajtai K, Borejdo J
Journal:Biochemistry
PubMed ID:16566579
Confocal microscopy is widely used for acquiring high spatial resolution tissue sample images of interesting fluorescent molecules inside cells. The fluorescent molecules are often tagged proteins participating in a biological function. The high spatial resolution of confocal microscopy compared to wide field imaging comes from an ability to optically isolate ... More