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Citas y referencias (15)
Invitrogen™
Glutathione Ethyl Ester, Biotin Amide (BioGEE) (Glutathiolation Detection Reagent) - Special Packaging
El éster etílico de glutatión biotinilado (BioGEE) es un análogo del glutatión biotinilado que penetra en la célula y queMás información
| Número de catálogo | Cantidad |
|---|---|
| G36000 | 10 x 100 μg |
Número de catálogo G36000
Precio (USD)
-
Cantidad:
10 x 100 μg
El éster etílico de glutatión biotinilado (BioGEE) es un análogo del glutatión biotinilado que penetra en la célula y que se utiliza para la detección de glutatiolación. En condiciones de estrés oxidativo, las células pueden de forma transitoria contener glutatión en las proteínas. Las células con estrés incubadas en BioGEE también incorporarán este derivado de glutatión biotinilado en las proteínas, lo que facilita la identificación de proteínas sensibles a la oxidación. Una vez que estas células están fijas y permeabilizadas, se pueden detectar los niveles de glutatiolación con un conjugado fluorescente de estreptavidina mediante citometría de flujo o microscopía de fluorescencia. Las proteínas glutatioladas con BioGEE también se pueden extraer y analizar mediante espectrometría de masas o con métodos Western blot junto con conjugados de estreptavidina marcados con fluoróforo o enzima.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Cantidad10 x 100 μg
Tipo de productoGlutathione Reagent
Unit SizeEach
Citations & References (15)
Citations & References
Abstract
Glutathione directly reduces an oxidoreductase in the endoplasmic reticulum of mammalian cells.
Journal:J Biol Chem
PubMed ID:15507438
'The formation of disulfide bonds is an essential step in the folding of many glycoproteins and secretory proteins. Non-native disulfide bonds are often formed between incorrect cysteine residues, and thus the cell has dedicated a family of oxidoreductases that are thought to isomerize non-native bonds. For an oxidoreductase to be
S-glutathiolation by peroxynitrite of p21ras at cysteine-118 mediates its direct activation and downstream signaling in endothelial cells.
Journal:FASEB J
PubMed ID:16415107
'The highly reactive species, peroxynitrite, is produced in endothelial cells in pathological states in which the production of superoxide anion and NO is increased. Here, we show that peroxynitrite added exogenously or generated endogenously in response to exposure to an NO donor or oxidized low-density lipoproteins (oxLDL) increases p21ras activity
Detection and affinity purification of oxidant-sensitive proteins using biotinylated glutathione ethyl ester.
Journal:Methods Enzymol
PubMed ID:12078486
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Detecting oxidative post-translational modifications in proteins.
Journal:Amino Acids
PubMed ID:17021655
Oxidative stress induces various post-translational modifications (PTM); some are reversible in vivo via enzymatic catalysis. The present paper reviews specific procedures for the detection of oxidative PTM in proteins, most of them including electrophoresis. Main topics are carbonylated and glutathionylated proteins as well as modification of selected amino acids (Cys,
Glutathione binding to the Bcl-2 homology-3 domain groove: a molecular basis for Bcl-2 antioxidant function at mitochondria.
Journal:J Biol Chem
PubMed ID:17690097
Bcl-2 protects cells against mitochondrial oxidative stress and subsequent apoptosis. However, the mechanism underlying the antioxidant function of Bcl-2 is currently unknown. Recently, Bax and several Bcl-2 homology-3 domain (BH3)-only proteins (Bid, Puma, and Noxa) have been shown to induce a pro-oxidant state at mitochondria (1-4). Given the opposing effects