HCS DNA Damage Kit
HCS DNA Damage Kit
Citas y referencias (15)
Invitrogen™

HCS DNA Damage Kit

El kit de daño de ADN de HCS mide simultáneamente y cuantitativamente dos parámetros importantes de salud celular: daño deMás información
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Número de catálogoCantidad
H102921 kit
Número de catálogo H10292
Precio (USD)
832,68
Each
Añadir al carro de la compra
Cantidad:
1 kit
Precio (USD)
832,68
Each
Añadir al carro de la compra
El kit de daño de ADN de HCS mide simultáneamente y cuantitativamente dos parámetros importantes de salud celular: daño de ADN y citotoxicidad. El daño de ADN se detecta utilizando un anticuerpo en comparación con el H2AX fosforilado (Ser139), que se induce en respuesta a la formación de rotura de doble cadena (DSB). Para detectar la citotoxicidad, Image-iT® DEAD™ Green es un tinte impermeable en las células sanas que se vuelve permeable cuando la membrana plasmática de las células se ve comprometida. Ambos indicadores son compatibles con la fijación y permeabilidad, permitiendo así el multiplexing con otros biomarcadores de interés. La herramienta de segmentación nuclear azul fluorescente Hoechst 33342 también se incluye y tiñe el ADN tanto en células vivas como muertas.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteAlexa Fluor™ 555, Hoechst 33258
FormatoPlaca de 96 pocillos
Cantidad1 kit
Condiciones de envíoHielo seco
ColorNaranja, azul, verde
Para utilizar con (equipo)Instrumentos de alto contenido
Línea de productosMolecular Probes
Tipo de productoKit de daños en ADN
Unit SizeEach
Contenido y almacenamiento
El kit contiene suficiente reactivo para procesar 2 placas de 96 pocillos.
  • Almacenar a ≤- 20 °C, desecar y proteger de la luz.

    • Preguntas frecuentes

    What are the fluorescence excitation/emission maxima for the dyes contained in the HCS DNA Damage Kit?

    Hoechst 33342: 350/461 nm, bound to DNA
    Image-IT Dead Green viability stain: 488/515 nm
    Alexa Fluor 555 goat anti-mouse IgG: 555/565 nm

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Citations & References (15)

    Citations & References
    Abstract
    Development of a high-content screening method for chemicals modulating DNA damage response.
    Authors:Kim S, Jun DH, Kim HJ, Jeong KC, Lee CH,
    Journal:J Biomol Screen
    PubMed ID:21233308
    The cellular response to DNA damage is emerging as a promising target for cancer therapy. In the present study, the authors exploited the relationship between the level of the phosphorylated form of histone H2AX (?H2AX) and the extent of DNA damage and developed a quantitative, cell-based, high-content screening system for ... More
    Induction of glutathione-dependent DNA double-strand breaks by the novel anticancer drug brostallicin.
    Authors:Guirouilh-Barbat J, Zhang YW, Pommier Y,
    Journal:Mol Cancer Ther
    PubMed ID:19584235
    Brostallicin is a DNA minor groove binder in phase II clinical trials. Here, we show that brostallicin induces gamma-H2AX nuclear foci that colocalize with 53BP1 and are dependent on glutathione, as shown by inhibition of those gamma-H2AX foci by l-buthionine sulfoximine. To differentiate brostallicin from the clinically approved minor groove ... More
    Free radical scavenging, a-glucosidase inhibitory and lipase inhibitory activities of eighteen Sudanese medicinal plants.
    Authors:Elbashir SMI, Devkota HP, Wada M, Kishimoto N, Moriuchi M, Shuto T, Misumi S, Kai H, Watanabe T,
    Journal:BMC Complement Altern Med
    PubMed ID:30340582
    'Lifestyle-related diseases such as diabetes are steadily increasing worldwide. In Sudan, there are a variety of plant species used traditionally for the treatment of diabetes, obesity and other symptoms which need to be validated through scientific studies for their claimed traditional uses. Therefore, in the current study, the free radical ... More
    Bioactive peptide amphiphile nanofiber gels enhance burn wound healing.
    Authors:Zhou S, Hokugo A, McClendon M, Zhang Z, Bakshi R, Wang L, Segovia LA, Rezzadeh K, Stupp SI, Jarrahy R
    Journal:Burns
    PubMed ID:31043333
    'Burns are physically debilitating and potentially fatal injuries. The standard-of-care for burn wounds is the coverage with gauze dressings designed to minimize trauma to the regenerating epidermis and dermis during dressing changes. However, deep partial- and full-thickness burns always heal slowly when standard wound care alone is performed. We have ... More
    A novel hydroxyphenyl hydrazone derivate YCL0426 inhibits cancer cell proliferation through sequestering iron.
    Authors:Li F, Long L, Xiao J, Wang C, Li W, Li S, Zhao C, Wang L
    Journal:Anticancer Drugs
    PubMed ID:28926421
    'Cancer cells have an increased requirement for iron than normal cells, and iron chelators are under active consideration for cancer treatment. The metal-sequestering potential and antiproliferative mechanisms of a novel hydroxyphenyl hydrazone derivate YCL0426 were investigated here. Antiproliferative activity of YCL0426 was detected by MTT assay. The iron-sequestering potential was ... More
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