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Citas y referencias (14)
Invitrogen™
Kit de ensayo de poros de transición mitocondrial Image-IT™ LIVE, para microscopía
El kit de ensayo de poros de transición mitocondrial Image-iT™ LIVE proporciona un método probado [Ref. PN36389, PN32679] para medirMás información
| Número de catálogo | Cantidad |
|---|---|
| I35103 | 1 Kit |
Número de catálogo I35103
Precio (USD)
1.091,88
Each
Cantidad:
1 Kit
Precio (USD)
1.091,88
Each
El kit de ensayo de poros de transición mitocondrial Image-iT™ LIVE proporciona un método probado [Ref. PN36389, PN32679] para medir la apertura de los poros de transición de permeabilidad mitocondrial. El kit incluye una tinción y un desactivador que se cargan fácilmente en las células. En las células sanas, la tinción entra en las mitocondrias, pero no el desactivador. Las mitocondrias permanecen fluorescentes hasta que la activación de los poros mitocondriales permite la desactivación de la fluorescencia.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorNaranja, azul, verde
Para utilizar con (equipo)Microscopio de fluorescencia
Línea de productosImage-iT
Tipo de productoKit de ensayo de poros de transición mitocondrial
Cantidad1 Kit
Condiciones de envíoTemperatura ambiente
Localización subcelularMitocondrias
FormatoKit
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.
Citations & References (14)
Citations & References
Abstract
Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence.
Journal:Biophys J
PubMed ID:9929477
'The occurrence and the mode of opening of the mitochondrial permeability transition pore (MTP) were investigated directly in intact cells by monitoring the fluorescence of mitochondrial entrapped calcein. When MH1C1 cells and hepatocytes were loaded with calcein AM, calcein was also present within mitochondria, because (i) its mitochondrial signal was
Effect of transient and permanent permeability transition pore opening on NAD(P)H localization in intact cells.
Journal:J Biol Chem
PubMed ID:19346250
'To study the effect of mitochondrial permeability transition pore (PTP) opening on NAD(P)H localization, intact cells were exposed to the Ca(2+) ionophore A23187. PTP opening, mitochondrial membrane potential, mitochondrial volume, and NAD(P)H localization were assessed by time-lapse laser confocal microscopy using the calcein-cobalt technique, tetramethylrhodamine methyl ester, MitoTracker, and NAD(P)H
Measuring apoptosis at the single cell level.
Journal:Methods
PubMed ID:18314052
The use of live cell microscopy has made a number of contributions to the study of apoptosis. Many of the tools and techniques are available that allow us to image the key events that occur during cell death including mitochondrial outer membrane permeabilization, mitochondrial transmembrane potential changes, translocation of Bcl-2
The mechanisms of apoptosis in biology and medicine: a new focus for ophthalmology.
Journal:Eur J Ophthalmol
PubMed ID:12749672
Defects in apoptosis (programmed cell death) have recently emerged as being closely involved in the pathogenesis of most ocular diseases and, therefore, apoptosis is now a topic of exponential interest in ophthalmology. This review summarizes recent works on mechanisms of apoptosis, from its initiation and modulation to the switching-on of
Therapeutic Potential of a Novel Necrosis Inhibitor, 7-Amino-Indole, in Myocardial Ischemia-Reperfusion Injury.
Journal:Hypertension
PubMed ID:29661840