Búfer de muestra LDS (4X), sin reducción

Número de catálogo	Cantidad
J61894.AD también denominado J61894-AD	50 mL

Número de catálogo J61894.AD

también denominado J61894-AD

Precio (USD)

Cantidad:

50 mL

Pedido a granel o personalizado

Especificaciones

ColorAzul

Almacenamiento recomendadoTemperaturas ambientes

Concentración4X

Forma físicaLíquido

Cantidad50 mL

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Aplicaciones
El tampón de muestra LDS (4X) no reductor se utiliza en la electroforesis en gel de SDS-poliacrilamida (SDS-PAGE). Se utiliza en geles de desnaturalización y es compatible con aplicaciones teñidas tanto con Coomassie y plata como con Western blotting.

Solubilidad
No miscible en agua.

Notas
Este tampón contiene: 988 mM de Tris-HCl, 2,04 mM de EDTA, 8 % de LDS, 40 % de glicerol, 0,88 % azul Coomassie y 0,7 mM rojo fenol con un pH 8,5.

RUO – Research Use Only

General References:

Yi Wang; Qiaozhen Lu; Shiaw-Lin Wu; Barry L. Karger and William S. Hancock. Characterization and Comparison of Disulfide Linkages and Scrambling Patterns in Therapeutic Monoclonal Antibodies: Using LC-MS with Electron Transfer Dissociation. Anal. Chem. 2011, 83, (8), 3133-3140.
Dan Lu; Zhenping Zhu. Construction and Production of an IgG-Like Tetravalent Bispecific Antibody for Enhanced Therapeutic Efficacy. Therapeutic Antibodies. 2008, 525, 377-404.

Preguntas frecuentes

At what temperature should I store LDS-sample buffer (4X), non-reducing (Cat. No. J61894.xx)?

LDS-sample buffer (4X), non-reducing should be stored at room temperature (20-25 degrees Celsius) upon receipt.

What protocol should I use for LDS-sample buffer (4X), non-reducing (Cat. No. J61894.xx)?

We recommend the following protocol for use of LDS-sample buffer (4X), non-reducing:
1. Mix one part LDS sample buffer to three parts protein (e.g., 5 µL sample buffer + 15 µL protein sample). Failure to properly dilute may result in a diffuse dye front.
2. Heat mixture at 100°C for 3-5 minutes.
3. Allow sample to cool back to room temperature.
4. Load sample onto SDS-polyacrylamide gel and begin electrophoresis.
5. Stop electrophoresis when the dye front nears the bottom of the gel.