Kit de expresión ViraPower™ Lentiviral Gateway™

Citas y referencias (11)

Invitrogen™

Kit de expresión ViraPower™ Lentiviral Gateway™

The ViraPower™ Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line,Más información

Número de catálogo	Cantidad
K496000	1 kit

Número de catálogo K496000

Precio (USD)

Cantidad:

1 kit

The ViraPower™ Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄V5-DEST™ vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.

Advantages
• Lentivirus based expression of a target gene in dividing and non-dividing mammalian cells

Key Features
• Flexible and versatile Gateway™ recombination cloning technology
• Constitutive high expression with CMV promoter
• Blasticidin selection marker for stable selection
• C terminal V5 tag for quick detection

Kit includes
• pLenti6⁄V5-DEST™ vector
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R70007)
• One Shot™ Stbl3™ Chemically Competent E. coli (C7373-03)

Related SKUs
• pLenti6⁄V5™ Directional TOPO™ Cloning Kit (K4955-10)
• ViraPower™ Lentiviral Directional TOPO™ Expression Kit (K4950-00)
• ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (K5330-00)
• ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit (K5310-00)

For research use only. Not intended for any therapeutic or diagnostic use.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Sistema constitutivo o inducibleConstitutivo

Tipo de entregaLentivírico

Para utilizar con (aplicación)Expresión viral

Tipo de productoKit de expresión lentiviral

Cantidad1 kit

Agente de selección (eucariótico)Blasticidina

VectorpLenti

Método de clonaciónGateway™

Línea de productosGateway, ViraPower

PromotorCMV

Etiqueta de proteínaEtiqueta de epítopo V5

Unit SizeEach

Contenido y almacenamiento

Kit de vectores pLenti6⁄V5-DEST™ Gateway™
Vectores pLenti6⁄V5™: -20 °C
E. coli químicamente competente One Shot™ Stbl3™: -80 °C

Kit de soporte lentiviral ViraPower™ Bsd
Mezcla de envasado ViraPower™: 20 °C
Lipofectamine™ 2000 +4 °C (NO congelar)
Blasticidina: -20 °C

Línea celular 293FT: nitrógeno líquido

Preguntas frecuentes

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Kit de expresión ViraPower™ Lentiviral Gateway™ FAQs

Citations & References (11)

Citations & References

Abstract

Folliculin encoded by the BHD gene interacts with a binding protein, FNIP1, and AMPK, and is involved in AMPK and mTOR signaling.

Authors:Baba M, Hong SB, Sharma N, Warren MB, Nickerson ML, Iwamatsu A, Esposito D, Gillette WK, Hopkins RF, Hartley JL, Furihata M, Oishi S, Zhen W, Burke TR, Linehan WM, Schmidt LS, Zbar B,

Journal:Proc Natl Acad Sci U S A

PubMed ID:17028174

'Birt-Hogg-Dubé syndrome, a hamartoma disorder characterized by benign tumors of the hair follicle, lung cysts, and renal neoplasia, is caused by germ-line mutations in the BHD(FLCN) gene, which encodes a tumor-suppressor protein, folliculin (FLCN), with unknown function. The tumor-suppressor proteins encoded by genes responsible for several other hamartoma syndromes, LKB1, ... More

GPCR screening via ERK 1/2: a novel platform for screening G protein-coupled receptors.

Authors:Osmond RI, Sheehan A, Borowicz R, Barnett E, Harvey G, Turner C, Brown A, Crouch MF, Dyer AR,

Journal:J Biomol Screen

PubMed ID:16129779

Discovery of novel agonists and antagonists for G protein-coupled receptors (GPCRs) relies heavily on cell-based assays because determination of functional consequences of receptor engagement is often desirable. Currently, there are several key parameters measured to achieve this, including mobilization of intracellular Ca2+ and formation of cyclic adenosine monophosphate or inositol ... More

Tumorigenesis suppressor Pdcd4 down-regulates mitogen-activated protein kinase kinase kinase kinase 1 expression to suppress colon carcinoma cell invasion.

Authors:Yang HS, Matthews CP, Clair T, Wang Q, Baker AR, Li CC, Tan TH, Colburn NH,

Journal:Mol Cell Biol

PubMed ID:16449643

Programmed cell death 4 (Pdcd4) suppresses neoplastic transformation by inhibiting the activation of c-Jun and consequently AP-1-dependent transcription. We report that Pdcd4 blocks c-Jun activation by inhibiting the expression of mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1)/hematopoietic progenitor kinase 1, a kinase upstream of Jun N-terminal kinase (JNK). cDNA ... More

Cutting Edge: Lentiviral short hairpin RNA silencing of PTEN in human mast cells reveals constitutive signals that promote cytokine secretion and cell survival.

Authors:Furumoto Y, Brooks S, Olivera A, Takagi Y, Miyagishi M, Taira K, Casellas R, Beaven MA, Gilfillan AM, Rivera J,

Journal:J Immunol

PubMed ID:16621980

Engagement of the FcepsilonRI expressed on mast cells induces the production of phosphatidylinositol 3, 4, 5-trisphosphate by PI3K, which is essential for the functions of the cells. PTEN (phosphatase and tensin homologue deleted on chromosome ten) directly opposes PI3K by dephosphorylating phosphatidylinositol 3, 4, 5-trisphosphate at the 3' position. In ... More

Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication.

Authors:Bruce JW, Bradley KA, Ahlquist P, Young JA,

Journal:J Virol

PubMed ID:16188999

In order to identify cellular proteins required for early stages of retroviral replication, a high volume screening with mammalian somatic cells was performed. Ten pools of chemically mutagenized Chinese hamster ovary (CHO-K1) cells were challenged with a murine leukemia virus (MLV) vector pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G), ... More

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