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Citas y referencias (23)
Invitrogen™
LI Silver (LIS) Enhancement Kit
El kit de mejora de plata de litio (LIS) es un sistema de mejora de plata práctico y no sensibleMás información
| Número de catálogo | Cantidad |
|---|---|
| L24919 | 1 kit |
Número de catálogo L24919
Precio (USD)
524,88
Each
Cantidad:
1 kit
Precio (USD)
524,88
Each
El kit de mejora de plata de litio (LIS) es un sistema de mejora de plata práctico y no sensible a la luz para usar con nuestros reactivos de oro coloidal y NANOGOLD. Las partículas de oro en presencia de iones de plata (I) y un agente reductor actúan como catalizadores para reducir los iones de plata (I) a plata metálica. La plata metálica se deposita en el oro, lo que amplia las partículas a entre 30 y 100 nm de diámetro. Los tejidos o blots teñidos con oro coloidal se “desarrollan” mediante este procedimiento autometalográfico para dar una tinción negra que puede verse en el microscopio óptico. El kit de mejora de plata de litio está preparado para realizar sondas moleculares por Nanoprobes, Inc.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Etiqueta o tinteNANOGOLD™, FluoroNanogold™, plata, oro coloidal
Tipo de productoKit de mejora de LIS
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8 °C) y proteger de la luz.
Citations & References (23)
Citations & References
Abstract
Immunolocalization of a Drosophila phosphatidylinositol transfer protein (rdgB) in normal and rdgA mutant photoreceptor cells with special reference to the subrhabdomeric cisternae.
Journal:J Electron Microsc (Tokyo)
PubMed ID:7996077
'Distribution of rdgB protein, which was recently shown to be a Drosophila phosphatidylinositol transfer protein, was studied in the photoreceptor cells of compound eyes of normal and rdgAPC47 mutant of Drosophila melanogaster by immunoelectron microscopy using (1) pre-embedding HRP staining, (2) pre-embedding NANOGOLD labeling followed by siliver enhancement, (3) and
A practical technique to postfix nanogold-immunolabeled specimens with osmium and to embed them in Epon for electron microscopy.
Journal:J Histochem Cytochem
PubMed ID:10727291
'Nanogold is a tiny gold probe, freely diffusible in cells and tissues, and is suitable for pre-embedding immunohistochemistry. However, it is necessary to develop Nanogold to a larger size so that it can be observed by conventional transmission electron microscopy. Silver enhancement is usually used for visualizing Nanogold, but the
Synaptotagmin VII as a plasma membrane Ca(2+) sensor in exocytosis.
Journal:Neuron
PubMed ID:11395007
'Synaptotagmins I and II are Ca(2+) binding proteins of synaptic vesicles essential for fast Ca(2+)-triggered neurotransmitter release. However, central synapses and neuroendocrine cells lacking these synaptotagmins still exhibit Ca(2+)-evoked exocytosis. We now propose that synaptotagmin VII functions as a plasma membrane Ca(2+) sensor in synaptic exocytosis complementary to vesicular synaptotagmins.
Cryo-electron microscopic localization of protein L7/L12 within the Escherichia coli 70 S ribosome by difference mapping and Nanogold labeling.
Journal:J Biol Chem
PubMed ID:11278411
'The Escherichia coli ribosomal protein L7/L12 is central to the translocation step of translation, and it is known to be flexible under some conditions. The assignment of electron density to L7/L12 was not possible in the recent 2.4 A resolution x-ray crystallographic structure (Ban, N., Nissen, P., Hansen, J., Moore,
Highly sensitive DNA, RNA and antigen detection methods: streptavidin-Nanogold-silver staining.
Journal:Cell Vis
PubMed ID:9660736