LIVE BacLight™ Bacterial Gram Stain Kit, for microscopy & quantitative assays
LIVE <i>Bac</i>Light&trade; Bacterial Gram Stain Kit, for microscopy &amp; quantitative assays
Citas y referencias (7)
Invitrogen™

LIVE BacLight™ Bacterial Gram Stain Kit, for microscopy & quantitative assays

El kit de tinción BacLight™ Bacterial Gram LIVE es un novedoso ensayo de fluorescencia en un solo paso para laMás información
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Número de catálogoCantidad
L70051 kit
Número de catálogo L7005
Precio (USD)
982,71
Each
Añadir al carro de la compra
Cantidad:
1 kit
Precio (USD)
982,71
Each
Añadir al carro de la compra
El kit de tinción BacLight™ Bacterial Gram LIVE es un novedoso ensayo de fluorescencia en un solo paso para la determinación de signos de Gram en bacterias vivas. Cuando las bacterias gramnegativas y grampositivas se tiñen simultáneamente con el colorante SYTO® 9 de membrana perpensada y el yoduro de hexidio, las bacterias gramnegativas se tiñen de verde fluorescente y las grampositivas de rojo fluorescente.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
DescripciónKit de tinción de Gram de bacterias LIVE BacLight™, para microscopía y ensayos cuantitativos
Método de detecciónFluorescente
Tipo de coloranteOtras etiquetas o colorantes
FormatoTubos, placa de 96 pocillos, portaobjetos
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
ColorVerde, rojo
Emission480-700 nm
Excitation Wavelength Range470 nm
Para utilizar con (aplicación)Ensayo de viabilidad
Para utilizar con (equipo)Microscopio de fluorescencia, Fluorímetro, Citómetro de flujo, Lector de microplacas
Línea de productosBacLight
Tipo de productoKit de tinción bacteriana
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the excitation/emission maxima for SYTO 9 stain and hexidium iodide?

The excitation/emission maxima for these dyes are 480/500 nm for SYTO 9 stain and 480/625 nm for hexidium iodide.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (7)

Citations & References
Abstract
Persistence and cell culturability of biocontrol strain Pseudomonas fluorescens CHA0 under plough pan conditions in soil and influence of the anaerobic regulator gene anr.
Authors:Mascher F, Schnider-Keel U, Haas D, Défago G, Moënne-Loccoz Y
Journal:Environ Microbiol
PubMed ID:12558593
'Certain fluorescent pseudomonads can protect plants from soil-borne pathogens, and it is important to understand how these biocontrol agents survive in soil. The persistence of the biocontrol strain Pseudomonas fluorescens CHA0-Rif under plough pan conditions was assessed in non-sterile soil microcosms by counting total cells (immunofluorescence microscopy), intact cells (BacLight ... More
Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.
Authors:Lebaron P, Parthuisot N, Catala P
Journal:Appl Environ Microbiol
PubMed ID:9572943
'Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4'',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial ... More
Determination of cell viability in single or mixed samples using capillary electrophoresis laser-induced fluorescence microfluidic systems.
Authors:Armstrong DW, He L
Journal:Anal Chem
PubMed ID:11605830
The advent of high-efficiency microbial separations will have a profound effect on both chemistry and microbiology. For the first time, it appears that it may be possible to obtain qualitative and quantitative information on microbial systems with the accuracy, precision, speed, and throughput that currently is found for chemical systems. ... More
Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters.
Authors:Durtschi JD, Erali M, Bromley LK, Herrmann MG, Petti CA, Smith RE, Voelkerding KV
Journal:J Med Microbiol
PubMed ID:16091435
A membrane-filter-based, fluorescent Gram stain method for bacterial detection in cerebrospinal fluid samples was developed and evaluated as a rapid, sensitive alternative to standard Gram stain protocols. A recently developed, modified version of the aluminium oxide membrane Anopore with low-fluorescence optical properties showed superior performance in this application. Other aspects ... More
Simultaneous fluorescent gram staining and activity assessment of activated sludge bacteria.
Authors:Forster S, Snape JR, Lappin-Scott HM, Porter J
Journal:Appl Environ Microbiol
PubMed ID:12324319
Wastewater treatment is one of the most important commercial biotechnological processes, and yet the component bacterial populations and their associated metabolic activities are poorly understood. The novel fluorescent dye hexidium iodide allows assessment of Gram status by differential absorption through bacterial cell walls. Differentiation between gram-positive and gram-negative wastewater bacteria ... More
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