Tampón de muestra TBE-urea Novex™ (2X)
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Invitrogen™

Tampón de muestra TBE-urea Novex™ (2X)

Para un rendimiento óptimo, se recomienda utilizar el tampón de muestras de TBE-urea Novex™ con los geles de TBE-urea Novex™.Más información
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Número de catálogoCantidad
LC687610 mL
Número de catálogo LC6876
Precio (USD)
101,91
Each
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Cantidad:
10 mL
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Precio (USD)
101,91
Each
Añadir al carro de la compra
Para un rendimiento óptimo, se recomienda utilizar el tampón de muestras de TBE-urea Novex™ con los geles de TBE-urea Novex™. Este tampón contiene urea y el agente de densidad Ficoll™, que produce bandas más nítidas y rectas que los agentes de densidad tradicionales, y los tintes de seguimiento azul de bromofenol y xileno cianol.

Acerca de los geles de TBE-urea Novex™
Los geles de TBE-urea de poliacrilamida desnaturalizante resuelven los oligonucleótidos de ADN monocatenario o el ARN en bandas nítidas y claras. Los geles de TBE-urea Novex™ están optimizados para el análisis y la purificación de productos que van desde 20 a 800 bases, lo que los convierte en una opción perfecta para el análisis y la purificación de oligonucleótidos sintéticos, ensayos de protección de ARNasa (RPA), estudios de transcripción in vitro y análisis mediante la técnica Northern. Los geles de TBE-urea Novex™ están diseñados para ejecutarse en la minicelda XCell SureLock™.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
TampónTampón de carga de muestras
Concentración2 X
Para utilizar con (aplicación)Electroforesis en gel de ácidos nucleicos, Blotting
Compatibilidad del gelGeles de poliacrilamida, geles de agarosa
Etiqueta o tinteAzul de bromofenol, xileno cianol
Línea de productosNovex
Tipo de productoTampón de muestras TBE
Cantidad10 mL
Condiciones de envíoHielo húmedo
Unit SizeEach
Contenido y almacenamiento
Almacenar a una temperatura de entre 2 y 8 °C

Preguntas frecuentes

My Novex TBE buffer has precipitated out of solution. What can I do?

If a slight turbidity develops, the fine precipitate can be dissolved by autoclaving for 5 minutes at 110°C. Do not autoclave in the container supplied. This treatment has no deleterious effect on the buffering properties of TBE.

Can a sample buffer with formamide be used with the Invitrogen TBE-Urea system?

There are many sample buffer formulations used, however we have found a distinct difference in the band appearance depending on the sample buffer composition. After evaluating urea, formamide, and various buffer systems, we found that the sharpest, flattest bands were obtained with a urea, Ficoll, and TBE buffer solution. Sample buffers made with formamide resulted in fuzzy, indistinct bands.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What can be used to stain Invitrogen precast TBE-Urea or TBE gels?

(1) Ethidium bromide: Soak gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 min. Destain by rinsing with three successive 10-min rinses of ultrapure water. Visualize bands under UV light. The gel must be removed from the cassette prior to visualization of the DNA under a UV light. Because polyacrylamide quenches the fluorescence of ethidium bromide, it is not possible to detect bands that contain less than about 10 ng of DNA by this method. SilverXpress and SYBR stains will provide greater detection sensitivity.

(2) Invitrogen SilverXpress Stain: Follow the standard procedure from the instruction booklet for staining TBE or TBE-Urea gels.

(3) SYBR Green I/II Nucleic Acid Gel Stain: See the SYBR Green Staining Manual for protocol details.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

(1) Ethidium bromide: Soak gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 min. Destain by rinsing with three successive 10-min rinses of ultrapure water. Visualize bands under UV light. The gel must be removed from the cassette prior to visualization of the DNA under a UV light. Because polyacrylamide quenches the fluorescence of ethidium bromide, it is not possible to detect bands that contain less than about 10 ng of DNA by this method. SilverXpress and SYBR stains will provide greater detection sensitivity.

(2) SilverXpress Stain: Follow the standard procedure from the instruction booklet for staining TBE or TBE-Urea gels.

(3) SYBR Green I Nucleic Acid Gel Stain (Cat. No. S7585)/SYBR Green II RNA Gel Stain (Cat. No. S7564): See the SYBR Green Staining Manual for protocol details.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.