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Citas y referencias (57)
Invitrogen™
Methotrexate, Fluorescein, triammonium Salt (Fluorescein Methotrexate)
El metotrexato de fluoresceína verde fluorescente (absorción⁄emisión maximas de ∼496⁄516 nm) se puede utilizar para sondear la expresión de dihidrofolatoMás información
| Número de catálogo | Cantidad |
|---|---|
| M1198MP | 1 mg |
Número de catálogo M1198MP
Precio (USD)
-
Cantidad:
1 mg
El metotrexato de fluoresceína verde fluorescente (absorción⁄emisión maximas de ∼496⁄516 nm) se puede utilizar para sondear la expresión de dihidrofolato reductasa (DHFR) mediante microscopía de fluorescencia o citometría de flujo.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de etiquetaColorantes clásicos
Fórmula molecularC46H54N14O9S
Cantidad1 mg
Almacenamiento recomendadoAlmacenar en el congelador (de -5 °C a -30 °C) y proteger de la luz.
Condiciones de envíoTemperatura ambiente
Forma físicaSólido
Tipo de productoSal de triamonio
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.
Citations & References (57)
Citations & References
Abstract
Xenobiotic transport across isolated brain microvessels studied by confocal microscopy.
Journal:Molecular pharmacology
PubMed ID:11093774
Visualization of membrane-associated folate transport proteins.
Journal:Adv Enzyme Regul
PubMed ID:2516406
'Transport of Methotrexate (MTX) into cells, via the "reduced folate" transport system, is a critical factor in the effectiveness of the drug in cancer chemotherapy, and defective transport is one of the principal types of resistance to MTX. Probes capable of detecting membrane-associated folate transport proteins (ftp''s) in individual cells
MRP2 and acquired tolerance to inorganic arsenic in the kidney of killifish (Fundulus heteroclitus).
Journal:Toxicol Sci
PubMed ID:17324950
'We used proximal tubules isolated from the killifish, Fundulus heteroclitus, to examine the effect of environmentally relevant, sublethal levels of arsenic on the function and expression of MRP2, an ABC transporter that transports xenobiotics into urine, including arsenic-glutathione conjugates. Exposure of fish to arsenic as sodium arsenite (4-14 days) increased
Characterization of a lipophilic antifolate resistance provoked by treatment of mammalian cells with the antiparasitic agent pyrimethamine.
Journal:J Biol Chem
PubMed ID:8440739
'We describe the characterization of an antitumor drug resistance following multiple step selection of hamster cells to the 2,4-diaminopyrimidines (DAP) metoprine, pyrimethamine (Pyr), and trimethoprim (Tmp). Pyr and Tmp are DAP lipophilic antifolates currently used as antiparasitic and antibacterial antibiotics, respectively. Dihydrofolate reductase (DHFR) from hamster cells bore a low
Defective transport as a mechanism of acquired resistance to methotrexate in patients with acute lymphocytic leukemia.
Journal:Blood
PubMed ID:1381235
'Although the mechanisms of resistance to methotrexate (MTX) are known in experimental tumors made resistant to this drug, little information is available regarding acquired resistance to MTX in patients. A competitive displacement assay using the fluorescent lysine analogue of MTX, N-(4-amino-4-deoxy-N10-methylpteroyl)-N epsilon-(4''-fluorescein-thiocarbamyl)-L-lysine (PT430), was developed as a sensitive method of