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Applied Biosystems™
MethylCode™ Bisulfite Conversion Kit
The MethylCode™ Bisulfite Conversion Kit is a fast and easy way to convert unmethylated cytosines from a DNA sample intoMás información
| Número de catálogo | Cantidad |
|---|---|
| MECOV50 | 50 reacciones |
Número de catálogo MECOV50
Precio (USD)
-
Cantidad:
50 reacciones
The MethylCode™ Bisulfite Conversion Kit is a fast and easy way to convert unmethylated cytosines from a DNA sample into uracils while methylated cytosines remain unchanged.
The modified DNA is ideal for PCR amplification for downstream analyses, including restriction endonuclease digestion, sequencing, and microarrays. With the MethylCode™ Bisulfite Conversion Kit you can expect:
–Simple, rapid cytosine methylation detection
–Consistency across most experimental conditions
–Streamlined conversion of unmethylated cytosines into uracil, thanks to a coupled DNA denaturation/bisulfite treatment step
–Ultra-pure DNA ideal for subsequent molecular-based analyses
Kit specifications:
–Protocol: Rapid heat denaturation of DNA followed by sodium bisulfite modification with spin column purification
–Sample sources: Plasmid, genomic, and endonuclease digested DNAs
–Input DNA: 500 pg-2 μg DNA per treatment, with 200-500 ng being optimal
–Recovery volume: Purified, bisulfite-treated DNA is recovered in 10 μl elution buffer
Contents and storage:
The MethylCode™ Bisulfite Conversion Kit contains all the reagents necessary to convert your DNA for methylation studies. The kit includes CT Conversion Reagent, Dilution Buffer, Resuspension buffer, Binding Buffer, Wash Buffer, Desulphonation Buffer, Elution Buffer, Spin Columns, Collection Tubes.
Store at room temperature for up to 24 months.
The modified DNA is ideal for PCR amplification for downstream analyses, including restriction endonuclease digestion, sequencing, and microarrays. With the MethylCode™ Bisulfite Conversion Kit you can expect:
–Simple, rapid cytosine methylation detection
–Consistency across most experimental conditions
–Streamlined conversion of unmethylated cytosines into uracil, thanks to a coupled DNA denaturation/bisulfite treatment step
–Ultra-pure DNA ideal for subsequent molecular-based analyses
Kit specifications:
–Protocol: Rapid heat denaturation of DNA followed by sodium bisulfite modification with spin column purification
–Sample sources: Plasmid, genomic, and endonuclease digested DNAs
–Input DNA: 500 pg-2 μg DNA per treatment, with 200-500 ng being optimal
–Recovery volume: Purified, bisulfite-treated DNA is recovered in 10 μl elution buffer
Contents and storage:
The MethylCode™ Bisulfite Conversion Kit contains all the reagents necessary to convert your DNA for methylation studies. The kit includes CT Conversion Reagent, Dilution Buffer, Resuspension buffer, Binding Buffer, Wash Buffer, Desulphonation Buffer, Elution Buffer, Spin Columns, Collection Tubes.
Store at room temperature for up to 24 months.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de productosMethylCode
Tipo de productoKit de conversión de bisulfito
Cantidad50 reacciones
Tipo de muestraADN (genómico)
Condiciones de envíoTemperatura ambiente
N.º de celda del material de partidaADN genómico purificado - 200 ng a 500 ng
FormatoKit
Unit SizeEach
Contenido y almacenamiento
El kit de conversión de bisulfito MethylCode™ contiene todos los reactivos necesarios para convertir su ADN para estudios de metilación. El kit incluye reactivo de conversión de TC, tampón de dilución, tampón de resuspensión, tampón de unión, tampón de lavado, tampón de desulfonación, tampón de elución, columnas de centrifugación y tubos de recogida.