NeuroTrace™ 640/660 Deep-Red Fluorescent Nissl Stain - Solution in DMSO
NeuroTrace™ 640/660 Deep-Red Fluorescent Nissl Stain - Solution in DMSO
Citas y referencias (20)
Invitrogen™

NeuroTrace™ 640/660 Deep-Red Fluorescent Nissl Stain - Solution in DMSO

La tinción de Nissl es el método histológico estándar para visualizar neuronas en cerebro y médula espinal Compuesto de ARNMás información
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Número de catálogoCantidad
N214831 mL
Número de catálogo N21483
Precio (USD)
442,26
Each
Añadir al carro de la compra
Cantidad:
1 mL
Precio (USD)
442,26
Each
Añadir al carro de la compra
La tinción de Nissl es el método histológico estándar para visualizar neuronas en cerebro y médula espinal Compuesto de ARN ribosomal asociado con el retículo endoplasmático áspero en pericaria neuronal y dendritas, la sustancia Nissl se redistribuye dentro del cuerpo celular en neuronas lesionadas o en regeneración, proporcionando un marcador del estado fisiológico de la neurona Nuestra tinción Nissl fluorescente rojo oscuro NeuroTrace 640/660 es un selectivo para la sustancia Nissl característica de las neuronas y proporciona más sensibilidad que los colorantes histológicos tradicionales, como el azul de toluidina o el violeta de cresilo
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorRojo oscuro
Método de detecciónFluorescencia
Para utilizar con (equipo)Microscopio de fluorescencia
Tipo de productoTinción de Nissl
Cantidad1 mL
Condiciones de envíoTemperatura ambiente
Localización subcelularCuerpos de Nissl
Excitation/Emission640/660 nm
Línea de productosNEUROTRACE
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I can use the NeuroTrace Nissl stains for staining glia or other cell types. What can I do to improve the staining so that it is more selective for neurons?

Our NeuroTrace Nissl stains label the Nissl substance which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells. These dyes are not completely specific for neurons, but will selectively stain neurons based on their high level of protein synthesis. In some cases they may show staining of other cell types such as glia, so you may need to decrease the staining concentration to obtain more selective neuronal labeling. We suggest dilutions in the range of 20- to 300-fold for neuronal staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the NeuroTrace Nissl stains be used on paraffin sections?

We have only tested them on mouse brain cryosections, however, there is at least one citation describing their use on paraffin tissue sections (Michelle L. Schlief, Ann Marie Craig, and Jonathan D. Gitlin. NMDA Receptor Activation Mediates Copper Homeostasis in Hippocampal Neurons. The Journal of Neuroscience, January 5, 2005, 25(1):239 - 246).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the NeuroTrace Nissl stains?

The dyes are proprietary, however they are stains that label the Nissl substance, which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (20)

Citations & References
Abstract
Glutamate acting on AMPA but not NMDA receptors modulates the migration of hippocampal interneurons.
Authors:Manent JB, Jorquera I, Ben-Ari Y, Aniksztejn L, Represa A,
Journal:J Neurosci
PubMed ID:16738232
'Paracrine GABA and glutamate acting, respectively, on GABAA and NMDA receptors modulate the migration of hippocampal pyramidal cells. Using corticohippocampal organotypic explants from glutamic acid decarboxylase (GAD) 67-enhanced green fluorescent protein (EGFP) knock-in embryos, we now report that, in contrast to pyramidal neurons, the blockade of AMPA but not NMDA ... More
Differential roles of the dopamine 1-class receptors, D1R and D5R, in hippocampal dependent memory.
Authors:Sariñana J, Kitamura T, Künzler P, Sultzman L, Tonegawa S,
Journal:
PubMed ID:24843151
'Activation of the hippocampal dopamine 1-class receptors (D1R and D5R) are implicated in contextual fear conditioning (CFC). However, the specific role of the D1R versus D5R in hippocampal dependent CFC has not been investigated. Generation of D1R- and D5R-specific in situ hybridization probes showed that D1R and D5R mRNA expression ... More
Phenotypic heterogeneity and plasticity of isocortical and hippocampal astrocytes in the human brain.
Authors:Sosunov AA, Wu X, Tsankova NM, Guilfoyle E, McKhann GM, Goldman JE,
Journal:
PubMed ID:24501367
'To examine the diversity of astrocytes in the human brain, we immunostained surgical specimens of temporal cortex and hippocampus and autopsy brains for CD44, a plasma membrane protein and extracellular matrix receptor. CD44 antibodies outline the details of astrocyte morphology to a degree not possible with glial fibrillary acidic protein ... More
Neutralization of Nogo-A enhances synaptic plasticity in the rodent motor cortex and improves motor learning in vivo.
Authors:Zemmar A, Weinmann O, Kellner Y, Yu X, Vicente R, Gullo M, Kasper H, Lussi K, Ristic Z, Luft AR, Rioult-Pedotti M, Zuo Y, Zagrebelsky M, Schwab ME,
Journal:
PubMed ID:24966370
The membrane protein Nogo-A is known as an inhibitor of axonal outgrowth and regeneration in the CNS. However, its physiological functions in the normal adult CNS remain incompletely understood. Here, we investigated the role of Nogo-A in cortical synaptic plasticity and motor learning in the uninjured adult rodent motor cortex. ... More
Superficially projecting principal neurons in layer V of medial entorhinal cortex in the rat receive excitatory retrosplenial input.
Authors:Czajkowski R, Sugar J, Zhang SJ, Couey JJ, Ye J, Witter MP,
Journal:
PubMed ID:24089485
Principal cells in layer V of the medial entorhinal cortex (MEC) have a nodal position in the cortical-hippocampal network. They are the main recipients of hippocampal output and receive inputs from several cortical areas, including a prominent one from the retrosplenial cortex (RSC), likely targeting basal dendrites of layer V ... More
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