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Invitrogen™
1,2-Dihexadecanoil-sn-glicero-3-fosfoetanolamina Oregon Green™ 488 (DHPE Oregon Green™ 488)
El fosfolípido, Oregon Green® 488 DHPE se etiqueta en el grupo funcional con el fluoróforo verde fluorescente Oregon Green® conMás información
| Número de catálogo | Cantidad |
|---|---|
| O12650 también denominado O-12650 | 1 mg |
Número de catálogo O12650
también denominado O-12650
Precio (USD)
-
Cantidad:
1 mg
El fosfolípido, Oregon Green® 488 DHPE se etiqueta en el grupo funcional con el fluoróforo verde fluorescente Oregon Green® con unos niveles máximos de excitación/emisión de ∼501/526 nm. Este analógico fluorado de la fluoresceína supera algunas de las limitaciones clave de la fluoresceína, incluyendo una mayor fotoestabilidad y un menor pKa (pKa ∼ 4,7 frente a 6,4 de la fluoresceína), haciendo su fluorescencia esencialmente insensible al pH en el rango de pH fisiológico.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Nombre del producto químico o materialFosfolípidos
Almacenamiento recomendadoAlmacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Forma físicaSolid
Línea de productosVerde Oregon
Cantidad1 mg
Unit SizeEach
Citations & References (7)
Citations & References
Abstract
Zero mode waveguides for single-molecule spectroscopy on lipid membranes.
Journal:Biophys J
PubMed ID:16461393
'Zero mode waveguides (ZMWs), subwavelength optical nanostructures with dimensions ranging from 50 to 200 nm, have been used to study systems involving ligand-receptor interactions. We show that under proper conditions, lipid membranes will invaginate into the nanostructures, which confine optical excitation to subattoliter volumes. Fluorescence correlation spectroscopy (FCS) was used
Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies.
Journal:EMBO J
PubMed ID:17159904
'SNARE proteins mediate membrane fusion in eukaryotic cells. They contain conserved SNARE motifs that are usually located adjacent to a C-terminal transmembrane domain. SNARE motifs spontaneously assemble into four helix bundles, with each helix belonging to a different subfamily. Liposomes containing SNAREs spontaneously fuse with each other, but it is
Surface-coupled proton exchange of a membrane-bound proton acceptor.
Journal:Proc Natl Acad Sci U S A
PubMed ID:20160117
Proton-transfer reactions across and at the surface of biological membranes are central for maintaining the transmembrane proton electrochemical gradients involved in cellular energy conversion. In this study, fluorescence correlation spectroscopy was used to measure the local protonation and deprotonation rates of single pH-sensitive fluorophores conjugated to liposome membranes, and the
Effects of linker sequences on vesicle fusion mediated by lipid-anchored DNA oligonucleotides.
Journal:Proc Natl Acad Sci U S A
PubMed ID:19164559
Synthetic lipid-oligonucleotide conjugates inserted into lipid vesicles mediate fusion when one population of vesicles displays the 5'-coupled conjugate and the other the 3'-coupled conjugate, so that anti-parallel hybridization allows the membrane surfaces to come into close proximity. Improved assays show that lipid mixing proceeds more quickly and to a much
Interplay between H1N1 influenza a virus infection, extracellular and intracellular respiratory tract pH, and host responses in a mouse model.
Journal:PLoS One
PubMed ID:33979408