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Invitrogen™
Pluronic™ F-127, baja absorbancia UV
Pluronic F-127 es un poliol surfactante no iónico (peso molecular aproximado de 12 500 daltons) que se ha observado queMás información
| Número de catálogo | Cantidad |
|---|---|
| P6867 | 2 g |
Número de catálogo P6867
Precio (USD)
78,14
Each
Cantidad:
2 g
Precio (USD)
78,14
Each
Pluronic F-127 es un poliol surfactante no iónico (peso molecular aproximado de 12 500 daltons) que se ha observado que facilita la solubilización de tintes no solubles en agua y de otros materiales en medios fisiológicos.1 Pluronic F-127 suele utilizarse para ayudar a dispersar los ésteres de acetoximetilo (AM) de nuestros indicadores de iones y tintes marcadores de células como el éster de carboxifluoresceína diacetato succinimidilo (CFDA-SE).
Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de membrana ›
Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de membrana ›
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Forma físicaPolvo
Almacenamiento recomendadoAlmacenar a temperatura ambiente.
Cantidad2 g
Unit SizeEach
Citations & References (141)
Citations & References
Abstract
Rescue of vasopressin V2 receptor mutants by chemical chaperones: specificity and mechanism.
Journal:Molecular biology of the cell
PubMed ID:16267275
Because missense mutations in genetic diseases of membrane proteins often result in endoplasmic reticulum (ER) retention of functional proteins, drug-induced rescue of their cell surface expression and understanding the underlying mechanism are of clinical value. To study this, we tested chemical chaperones and sarco(endo)plasmic reticulum Ca(2+) ATPase pump inhibitors on
Indo-1 binding to protein in permeabilized ventricular myocytes alters its spectral and Ca binding properties.
Journal:Biophys J
PubMed ID:1420876
We have examined the binding of the fluorescent Ca indicator indo-1 to cellular protein in permeabilized ventricular myocytes and also to soluble and particulate myocyte protein. Using either a filtration technique or equilibrium dialysis, and conditions similar to those in a cardiac myocyte patch clamped with 100 microM indo-1 in
Long-term regulation of neuronal calcium currents by prolonged changes of membrane potential.
Journal:J Neurosci
PubMed ID:1315850
'Although rapid-onset, short-term regulation of neuronal Ca currents by neurotransmitters and second messengers is well documented, little is known about conditions that can cause longer-lasting changes in Ca channel function. We report here that persistent depolarization is accompanied by slowly developing long-term reduction of neuronal Ca currents. Rat myenteric neurons
Regulation of cytosolic free calcium concentration by intrasynaptic mitochondria.
Journal:Mol Biol Cell
PubMed ID:1550964
'By the use of digitonin permeabilized presynaptic nerve terminals (synaptosomes), we have found that intrasynaptic mitochondria, when studied "in situ," i.e., surrounded by their cytosolic environment, are able to buffer calcium in a range of calcium concentrations close to those usually present in the cytosol of resting synaptosomes. Adenine nucleotides
Intracellular astrocyte calcium waves in situ increase the frequency of spontaneous AMPA receptor currents in CA1 pyramidal neurons.
Journal:J Neurosci
PubMed ID:14736858
'Spontaneous neurotransmitter release and activation of group I metabotropic glutamate receptors (mGluRs) each play a role in the plasticity of neuronal synapses. Astrocytes may contribute to short- and long-term synaptic changes by signaling to neurons via these processes. Spontaneous whole-cell AMPA receptor (AMPAR) currents were recorded in CA1 pyramidal cells