PS-Speck™ Microscope Point Source Kit (blue, green, orange & deep-red fluorescent beads)
PS-Speck™ Microscope Point Source Kit (blue, green, orange & deep-red fluorescent beads)
Invitrogen™

PS-Speck™ Microscope Point Source Kit (blue, green, orange & deep-red fluorescent beads)

El kit de fuente puntual de microscopio PS-Speck™ contiene cuatro colores diferentes de microesferas de fluorescencia; cada microesfera tiene unMás información
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Número de catálogoCantidad
P72201 kit
Número de catálogo P7220
Precio (USD)
417,85
Each
Añadir al carro de la compra
Cantidad:
1 kit
Precio (USD)
417,85
Each
Añadir al carro de la compra
El kit de fuente puntual de microscopio PS-Speck™ contiene cuatro colores diferentes de microesferas de fluorescencia; cada microesfera tiene un diámetro de 0,175 +/- 0,005 µm. Este coeficiente de variación de diámetro excepcionalmente pequeño hace que las microesferas PS-Speck™ sean ideales como fuentes puntuales de fluorescencia y de subresolución uniformes para calibrar la óptica de los instrumentos, especialmente en aplicaciones de adquisición de imágenes tridimensionales. Las cuatro suspensiones de 1 ml listas para usar de este kit contienen microesferas brillantes monodispersas con longitudes de onda de excitación/emisión de 360/440 nm (azul), 505/515 nm (verde), 540/560 nm (naranja) y 633/660 nm (rojo oscuro). El kit PS-Speck™ también incluye un protocolo de montaje para la comodidad del usuario y un medio de montaje suficiente para preparar unos 100 portaobjetos de cada uno.

Consulte nuestra colección completa de reactivos de calibración para microscopios ›

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de calibraciónCalibración óptica, calibración de microscopio de fluorescencia
Para utilizar con (aplicación)Patrones de microscopía de fluorescencia interna o externa
FormatoGránulos de suspensión
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
ColorNaranja, rojo oscuro, azul, verde
Línea de productosPS-Speck
TipoKit de fuente puntual de microscopio
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

What is the refractive index of the mounting medium in PS-Speck Microscope Point Source Kit (Cat. No. P7220)?

The mountant (Component E) has a refractive index of ~1.47.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (13)

Citations & References
Abstract
Correcting confocal acquisition to optimize imaging of fluorescence resonance energy transfer by sensitized emission.
Authors:van Rheenen J, Langeslag M, Jalink K
Journal:Biophys J
PubMed ID:15041688
Imaging of fluorescence resonance energy transfer (FRET) between suitable fluorophores is increasingly being used to study cellular processes with high spatiotemporal resolution. The genetically encoded Cyan (CFP) and Yellow (YFP) variants of Green Fluorescent Protein have become the most popular donor and acceptor pair in cell biology. FRET between these ... More
In vivo mammalian brain imaging using one- and two-photon fluorescence microendoscopy.
Authors:Jung JC, Mehta AD, Aksay E, Stepnoski R, Schnitzer MJ
Journal:J Neurophysiol
PubMed ID:15128753
'One of the major limitations in the current set of techniques available to neuroscientists is a dearth of methods for imaging individual cells deep within the brains of live animals. To overcome this limitation, we developed two forms of minimally invasive fluorescence microendoscopy and tested their abilities to image cells ... More
Interaction of Maf transcription factors with Pax-6 results in synergistic activation of the glucagon promoter.
Authors:Planque N, Leconte L, Coquelle FM, Benkhelifa S, Martin P, Felder-Schmittbuhl MP, Saule S
Journal:J Biol Chem
PubMed ID:11457839
'In the endocrine pancreas, alpha-cell-specific expression of the glucagon gene is mediated by DNA-binding proteins that interact with the G1 proximal promoter element. Among these proteins, the paired domain transcription factor Pax-6 has been shown to bind to G1 and to transactivate glucagon gene expression. Close to the Pax-6-binding site, ... More
Quality assessment of confocal microscopy slide based systems: performance.
Authors:Zucker RM
Journal:Cytometry A
PubMed ID:16807897
'BACKGROUND: All fluorescence slide-based cytometry detections systems basically include the following components: (1) an excitation light source, (2) intermediate optics, and (3) a detection device consisting of a CCD camera or a PMT. The optical principles employed is slide-based systems are similar to those of confocal microscopes (CLSM). METHODS: The ... More
Spatial organization of bacteriorhodopsin in model membranes. Light-induced mobility changes.
Authors:Kahya N, Wiersma DA, Poolman B, Hoekstra D
Journal:J Biol Chem
PubMed ID:12167614
'Bacteriorhodopsin is a proton-transporting membrane protein in Halophilic archaea, and it is considered a prototype of membrane transporters and a model for G-protein-coupled receptors. Oligomerization of the protein has been reported, but it is unknown whether this feature is correlated with, for instance, light activation. Here, we have addressed this ... More