Phenolic Acridine Orange Decolorizer

Use Thermo Scientific™ Remel™ Phenolic Acridine Orange Decolorizer along with Phenolic Acridine Orange to detect acid-fast bacilli in clinical specimens. In 1977, Kronvall and Myhre described the use of acridine orange stain to detect microorganisms in direct smears prepared from clinical specimens¹. In 1955, Smithwick et al. described an acid-fast method which incorporated an acridine orange solution that contained phenol². Phenolic Acridine Orange stain was found to produce less nonspecific fluorescence of background material. This makes it superior to auramine O.

Phenolic Acridine Orange is a stain which penetrates the mycobacterial cell wall and binds to the nucleic acid of bacteria and other cells, either in the native or the denatured state. Methylene blue that has been added to Phenolic Acridine Orange decolorizer reduces background fluorescence in stained smears.

• Ready-to-use – Liquid reagent
• Easy-to-observe – Examine smears with a fluorescent microscope with 200 X magnification and confirm the morphology with 600 X magnification.
• Easy-to-interpret – Positive test shows bright-red to orange fluorescent bacilli with characteristic morphology confirmed at 600 X magnification. In case of negative test no bright-red to orange fluorescent bacilli observed after examination of 30 fields at 200 X magnification.

Phenolic Acridine Orange Decolorizer contains concentrated hydrochloric acid (CAS 7647-01-0), 95% ethyl alcohol (CAS 64-17-5), demineralized water (CAS 7732-18-5) and methylene blue (CAS 61-73-4).

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.