Células FreeStyle™ 293F
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Citas y referencias (3)
Gibco™

Células FreeStyle™ 293F

La estirpe de células FreeStyle™ 293-F deriva de la estirpe de células 293 y se destina al uso con elMás información
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Número de catálogoCantidad
R790071 ml
Número de catálogo R79007
Precio (USD)
-
Cantidad:
1 ml
La estirpe de células FreeStyle™ 293-F deriva de la estirpe de células 293 y se destina al uso con el sistema de expresión FreeStyle™ MAX 293 o el sistema de expresión FreeStyle™ 293. Las células FreeStyle™ 293-F se han adaptado al cultivo en suspensión en el medio de expresión FreeStyle™ 293. Se suministran células congeladas que se pueden descongelar directamente en el medio de expresión FreeStyle™ 293. La estirpe de células FreeStyle™ 293-F se suministra en un vial que contiene 1 ml de células a 1 × 107 células viables/ml en el medio de expresión FreeStyle™ 293 al 90 % y dimetilsulfóxido (DMSO) al 10 %. Características de las células FreeStyle™ 293-F:

• Preparadas a partir de cultivos de bancos de células Master de pase bajo derivadas de las células 293-F parentales que se han vuelto a clonar limitando la clonación. Los cultivos clonales derivados de 293 se mantienen en condiciones sin suero en solo de 30 a 35 de los pases totales.
• Adaptadas al crecimiento en suspensión de alta densidad sin sueros. Se pueden mantener en el medio de expresión FreeStyle™ 293.
• Demuestran una transfección de gran eficacia con el reactivo FreeStyle™ MAX.
• Los cultivos en suspensión se pueden transfectar en el medio de expresión FreeStyle™ 293 sin necesidad de cambiar los medios.
• Permite la transfección de células en grandes volúmenes.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de célulasCélulas FreeStyle™ 293F
Para utilizar con (aplicación)Expresión y transfección de proteínas
Adaptación de los mediosMedio de expresión Freestyle™ 293
Línea de productosFreestyle
Tipo de productoCélula 293-F
Cantidad1 ml
Tipo de sistemaFreestyle™
Tipo de célula293-F
EspecieHumano
Unit SizeEach
Contenido y almacenamiento
1 vial de 1 × 107 células Almacenar en nitrógeno líquido.

Preguntas frecuentes

I do not have FreeStyle 293-F cells. Can I use a different 293 cell line with the FreeStyle 293 Expression System?

Other 293 cell lines may be used with the FreeStyle 293 Expression System. However, they would have to be adapted to serum-free suspension culture in FreeStyle 293 Expression Medium and evaluated for transfection and expression.

How do FreeStyle 293-F cells compare to FreeStyle CHO-S cells in terms of antibody and protein yield?

When comparing FreeStyle 293-F cells with FreeStyle CHO-S cells for average range of antibody and protein yield, it is definitely expected that antibody yield will be higher in FreeStyle 293-F cells than in FreeStyle CHO-S cells, when generated by transient transfection. On the other hand, antibody yields in stable CHO cells can be much higher. For non-antibody proteins, it is impossible to predict which of the two cell lines may provide higher yields.

Can FreeStyle 293-F cells grown in FreeStyle 293 expression medium be transfected with ExpiFectamine293 Reagent along with enhancers? Is so, will the transfection efficiency be higher than that with FreeStyle Max Reagent?

You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How long can I keep culturing FreeStyle cells after transfection?

We typically keep the culture going for 7-10 days posttransfection. After that, protein expression may drop.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to grow FreeStyle 293-F cells in 8% CO2 when using the FreeStyle 293 Expression Medium? If 8% CO2 cannot be arranged, can I supplement the media with an additional reagent?

Our recommendation of 8% CO2 for the optimal growth of Freestyle 293 cells in Freestyle 293 Expression Medium is based on the bicarbonate content in the media and growth conditions of the cells observed by R&D. That said, you may use lower concentrations of CO2 if necessary (5% is common) to grow these cells. You may observe slightly slower growth kinetics or lower cell concentrations, but functionally the entire system will still work.

View all Células FreeStyle™ 293F FAQs

Citations & References (3)

Citations & References
Abstract
Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10.
Authors:Ido H, Harada K, Futaki S, Hayashi Y, Nishiuchi R, Natsuka Y, Li S, Wada Y, Combs AC, Ervasti JM, Sekiguchi K,
Journal:J Biol Chem
PubMed ID:14701821
'The adhesive interactions of cells with laminins are mediated by integrins and non-integrin-type receptors such as alpha-dystroglycan and syndecans. Laminins bind to these receptors at the C-terminal globular domain of their alpha chains, but the regions recognized by these receptors have not been mapped precisely. In this study, we sought ... More
Biological activity of follistatin isoforms and follistatin-like-3 is dependent on differential cell surface binding and specificity for activin, myostatin, and bone morphogenetic proteins.
Authors:Sidis Y, Mukherjee A, Keutmann H, Delbaere A, Sadatsuki M, Schneyer A,
Journal:Endocrinology
PubMed ID:16627583
Follistatin (FST) and FST-like-3 (FSTL3) are activin-binding and neutralization proteins that also bind myostatin. Three FST isoforms have been described that differ in tissue distribution and cell-surface binding activity, suggesting that the FST isoforms and FSTL3 may have some nonoverlapping biological actions. We produced recombinant FST isoforms and FSTL3 and ... More
Mutation of key residues of RPE65 abolishes its enzymatic role as isomerohydrolase in the visual cycle.
Authors:Redmond TM, Poliakov E, Yu S, Tsai JY, Lu Z, Gentleman S,
Journal:Proc Natl Acad Sci U S A
PubMed ID:16150724
RPE65 is essential for isomerization of vitamin A to the visual chromophore. Mutations in RPE65 cause early-onset blindness, and Rpe65-deficient mice lack 11-cis-retinal but overaccumulate alltrans-retinyl esters in the retinal pigment epithelium (RPE). RPE65 is proposed to be a substrate chaperone but may have an enzymatic role because it is ... More