Tinción para gel de ácido nucleico SYBR™ Green I, 10 000X concentrado en DMSO
Tinción para gel de ácido nucleico SYBR™ Green I, 10 000X concentrado en DMSO
Citas y referencias (455)
Invitrogen™

Tinción para gel de ácido nucleico SYBR™ Green I, 10 000X concentrado en DMSO

La tinción en geles de ácido nucleico SYBR™ Green I es una de las coloraciones más sensibles que se ofrecenMás información
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Número de catálogoCantidad
S75671 ml
S7563500 μl
S758520 x 50 μl
Número de catálogo S7567
Precio (USD)
993,30
Each
Añadir al carro de la compra
Cantidad:
1 ml
Pedido a granel o personalizado
Precio (USD)
993,30
Each
Añadir al carro de la compra
La tinción en geles de ácido nucleico SYBR™ Green I es una de las coloraciones más sensibles que se ofrecen para detectar ADN bicatenario (ADNbc) en geles de agarosa y poliacrilamida. Como SYBR™ Green I tiene una mayor sensibilidad para el ADNbc, resulta especialmente útil para ensayos en los que la presencia de ADNmc o ARN contaminante podría ocultar los resultados. Gracias a una fluorescencia con un fondo excepcionalmente bajo y características espectrales que se aproximan en gran medida a las fuentes de luz y los conjuntos de filtros de los instrumentos existentes, la tinción SYBR™ Green I es ideal para su uso con lectores láser. La tinción SYBR™ Green I ofrece:

• Sensibilidad: por lo menos cuatro veces más grande que la del bromuro de etidio para ADN en geles de agarosa
• Facilidad de uso: los geles empapados en una tinción diluida se pueden visualizar sin desalinización
• Compatibilidad: con transiluminadores UV, sistemas de documentación de geles y escáneres láser
• Flexibilidad: para su uso con una amplia gama de aplicaciones, incluida la tipificación de ADN, los ensayos basados en PCR, los ensayos de daños en el ADN, el análisis de muestras complejas y la detección de PCR en tiempo real

Uso del producto
1 ml permite teñir 100 minigeles. Los paquetes están disponibles en diversos tamaños.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Ubicación de detecciónDetección en gel
Método de detecciónFluorescencia
Tipo de productoTinción para geles de ácidos nucleicos
Cantidad1 ml
Condiciones de envíoTemperatura ambiente
Molécula dianaADN
Etiqueta o tinteSYBR Green I
Unit SizeEach
Contenido y almacenamiento
• Se suministra como concentrado de 10 000X en DMSO

Almacenar a -20 °C, proteger de la luz en un desecador.

Preguntas frecuentes

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the recommended filter for my gel documentation system?

Please go here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html) and click on the “Filter Recommendations” tab to see filter recommendations for use with SYBR Safe DNA Gel Stain. Note that the excitation and emission spectra of SYBR Safe DNA Gel Stain are very similar to those of SYBR Green I, SYBR Green II, and SYBR Gold dyes, as well as fluorescein (FITC). Therefore, filters appropriate for these dyes can also be used. A camera filter is not required with the Safe Imager Blue-Light Transilluminator; the amber filter provided with the instrument serves this purpose.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I use the ethidium bromide filters on my camera to image SYBR dyes?

This is not recommended. Most deep amber/orange ethidium bromide filters have a cutoff value around 550 nm. The SYBR Green dyes emit at 520 nm, which will not be detected using this filter.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Is SYBR Safe DNA Gel Stain the same as SYBR Green I dye?

All SYBR dyes have similar spectral properties, but have different chemical compositions. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain (Cat. No. S33102) was specifically developed as a safer alternative to ethidium bromide. SYBR Green I (Cat. No. S7585) is an ultrasensitive stain for dsDNA, and SYBR Green II (Cat. No. S7564) is a highly sensitive stain for RNA and ssDNA. All SYBR dyes are optimally excited by the Safe Imager Blue-Light Transilluminator.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

How should I dispose of SYBR DNA Gel Stain?

Disposal regulations vary. Please contact your safety office or local municipality for disposal guidelines.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

View all SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in DMSO, 1 mL FAQs

Citations & References (455)

Citations & References
Abstract
A rapid and sensitive approach to mutation detection using real-time polymerase chain reaction and melting curve analyses, using BRCA1 as an example.
Authors:Pals G,Pindolia K,Worsham MJ
Journal:Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology
PubMed ID:10553024
Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature.
Authors:Gudnason H,Dufva M,Bang DD,Wolff A
Journal:Nucleic acids research
PubMed ID:17897966
The importance of real-time polymerase chain reaction (PCR) has increased steadily in clinical applications over the last decade. Many applications utilize SYBR Green I dye to follow the accumulation of amplicons in real time. SYBR Green I has, however, a number of limitations that include the inhibition of PCR, preferential ... More
Diagnosis of herpes simplex virus infections in the clinical laboratory by LightCycler PCR.
Authors:Espy MJ,Uhl JR,Mitchell PS,Thorvilson JN,Svien KA,Wold AD,Smith TF
Journal:Journal of clinical microbiology
PubMed ID:10655387
Herpes simplex virus (HSV) causes several clinical manifestations in both normal and immunocompromised hosts; this agent is the most frequently detected virus in diagnostic laboratories. Recovery of the virus in cell culture is considered the “gold standard” for detection of this virus from sources other than cerebrospinal fluid. LightCycler is ... More
Kruppel-like factor 2 (KLF2) regulates proinflammatory activation of monocytes.
Authors:Das H,Kumar A,Lin Z,Patino WD,Hwang PM,Feinberg MW,Majumder PK,Jain MK
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:16617118
The mechanisms regulating activation of monocytes remain incompletely understood. Herein we provide evidence that Kruppel-like factor 2 (KLF2) inhibits proinflammatory activation of monocytes. In vitro, KLF2 expression in monocytes is reduced by cytokine activation or differentiation. Consistent with this observation, KLF2 expression in circulating monocytes is reduced in patients with ... More
Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.
Authors:Plaia TW,Josephson R,Liu Y,Zeng X,Ording C,Toumadje A,Brimble SN,Sherrer ES,Uhl EW,Freed WJ,Schulz TC,Maitra A,Rao MS,Auerbach JM
Journal:Stem cells (Dayton, Ohio)
PubMed ID:16293579
View all SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in DMSO, 1 mL citations