Colorante JC-1 (sonda potencial de membrana mitocondrial)

Citas y referencias (400)

Invitrogen™

Colorante JC-1 (sonda potencial de membrana mitocondrial)

JC-1 es un novedoso colorante de carbocianina catiónica que se acumula en la mitocondria. El tinte existe como un monómeroMás información

Número de catálogo	Cantidad
T3168	5 mg

Número de catálogo T3168

Precio (USD)

865,20

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Cantidad:

Precio (USD)

865,20

Añadir al carro de la compra

JC-1 es un novedoso colorante de carbocianina catiónica que se acumula en la mitocondria. El tinte existe como un monómero en bajas concentraciones y produce una fluorescencia verde, similar a la fluoresceína. En concentraciones superiores, el colorante forma agregados J que presentan un amplio espectro de excitación y una emisión máxima a ∼590 nm. Estas características convierten al JC-1 en un marcador sensible para el potencial de membrana mitocondrial. Otro tinte con similares características es el JC-9 (D-22421).

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Método de detecciónFluorescente

Cantidad5 mg

Condiciones de envíoTemperatura ambiente

Localización subcelularMitocondrias

ColorVerde

Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo

Tipo de productoTinte

Unit Size5 mg

Contenido y almacenamiento

Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (400)

Citations & References

Abstract

Apoptosis induced by Rac GTPase correlates with induction of FasL and ceramides production.

Authors:Embade N,Valerón PF,Aznar S,López-Collazo E,Lacal JC

Journal:Molecular biology of the cell

PubMed ID:11102528

Rho proteins, members of the Ras superfamily of GTPases, are critical elements in signal transduction pathways governing cell proliferation and cell death. Different members of the family of human Rho GTPases, including RhoA, RhoC, and Rac1, participate in the regulation of apoptosis in response to cytokines and serum deprivation in ... More

Large-scale chemical dissection of mitochondrial function.

Authors:Wagner BK,Kitami T,Gilbert TJ,Peck D,Ramanathan A,Schreiber SL,Golub TR,Mootha VK

Journal:Nature biotechnology

PubMed ID:18297058

Mitochondrial oxidative phosphorylation (OXPHOS) is central to physiology and disease pathogenesis. To systematically investigate its activity and regulation, we performed a wide range of assays of OXPHOS physiology and nuclear and mitochondrial gene expression across 2490 chemical perturbations in muscle cells. Through mining of the resulting compendium, we discovered that: ... More

Authors:

Journal:

PubMed ID:10891486

Mitochondria--potential role in cell life and death.

Authors:Griffiths EJ

Journal:Cardiovascular research

PubMed ID:10727650

The mitochondrial death/life regulator in apoptosis and necrosis.

Authors:Kroemer G,Dallaporta B,Resche-Rigon M

Journal:Annual review of physiology

PubMed ID:9558479

Both physiological cell death (apoptosis) and, in some cases, accidental cell death (necrosis) involve a two-step process. At a first level, numerous physiological and some pathological stimuli trigger an increase in mitochondrial membrane permeability. The mitochondria release apoptogenic factors through the outer membrane and dissipate the electrochemical gradient of the ... More

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