Solución de etiquetado celular Vybrant™ DiD
Solución de etiquetado celular Vybrant™ DiD
Citas y referencias (24)
Invitrogen™

Solución de etiquetado celular Vybrant™ DiD

La solución de etiquetado de células Vybrant™ DiD es una solución de suministro de colorante que se puede añadir directamenteMás información
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Número de catálogoCantidad
V228871 mL
Número de catálogo V22887
Precio (USD)
302,94
1 mL
Añadir al carro de la compra
Cantidad:
1 mL
Precio (USD)
302,94
1 mL
Añadir al carro de la compra
La solución de etiquetado de células Vybrant™ DiD es una solución de suministro de colorante que se puede añadir directamente a medios de cultivos normales para etiquetar de manera uniforme células de cultivos adheridas o de suspensión para su uso en aplicaciones de fusión intercelular, adhesión celular y migración.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorRojo
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia, citómetro de flujo
Línea de productosVybrant
Cantidad1 mL
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaFluorescent Dye
Tipo de productoSolución de etiquetado celular
SubCellular LocalizationMembranas celulares y lípidos, Lipids
Unit Size1 mL
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to perform a cell fusion assay, where one cell line is labeled with one color and the other cell line with another color, and combine with a nucleic acid stain. What do you recommend?

A typical method is to label one cell line with orange fluorescent DiI C18 and the other cell line with green fluorescent DiO C18. These orange and green lipophilic cyanine dyes will stain the membranes of cells. Cells that fuse will then have both dyes, yielding a yellow color (when images are overlaid or cells are imaged in a dual-bandpass filter). These live cells can then be labeled with Hoechst 33342 (a cell-permeant blue DNA stain comparable in wavelength to DAPI), but only as an endpoint just before imaging (since DNA stains can interrupt DNA function).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to look at live cell morphology deformation over the course of a few hours. What sort of membrane dye would be useful for this?

Lipophilic cyanine dyes, such as DiI (Cat. No. D282), DiO (Cat. No. D275), DiD (Cat. No. D7757) or DiR (Cat. No. D12731), are commonly used. The longer the alkyl chain on the dye, the better the retention in lipophilic environments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (24)

Citations & References
Abstract
Gap junction adhesion is necessary for radial migration in the neocortex.
Authors:Elias LA, Wang DD, Kriegstein AR,
Journal:Nature
PubMed ID:17713529
'Radial glia, the neuronal stem cells of the embryonic cerebral cortex, reside deep within the developing brain and extend radial fibres to the pial surface, along which embryonic neurons migrate to reach the cortical plate. Here we show that the gap junction subunits connexin 26 (Cx26) and connexin 43 (Cx43) ... More
Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1.
Authors:Gazit R, Gruda R, Elboim M, Arnon TI, Katz G, Achdout H, Hanna J, Qimron U, Landau G, Greenbaum E, Zakay-Rones Z, Porgador A, Mandelboim O
Journal:Nat Immunol
PubMed ID:16565719
The elimination of viruses and tumors by natural killer cells is mediated by specific natural killer cell receptors. To study the in vivo function of a principal activating natural killer cell receptor, NCR1 (NKp46 in humans), we replaced the gene encoding this receptor (Ncr1) with a green fluorescent protein reporter ... More
Cell tracking with optical imaging.
Authors:Sutton EJ, Henning TD, Pichler BJ, Bremer C, Daldrup-Link HE,
Journal:Eur Radiol
PubMed ID:18506449
Adaptability, sensitivity, resolution and non-invasiveness are the attributes that have contributed to the longstanding use of light as an investigational tool and form the basis of optical imaging (OI). OI, which encompasses numerous techniques and methods, is rapid (<5 min), inexpensive, noninvasive, nontoxic (no radiation) and has molecular (single-cell) sensitivity, ... More
Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells.
Authors:Shalek AK, Robinson JT, Karp ES, Lee JS, Ahn DR, Yoon MH, Sutton A, Jorgolli M, Gertner RS, Gujral TS, Macbeath G, Yang EG, Park H,
Journal:Proc Natl Acad Sci U S A
PubMed ID:20080678
A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, and universal delivery of biomolecules into immortalized and primary mammalian cells using surface-modified vertical silicon nanowires. The method relies ... More
Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophils.
Authors:Wang F, Herzmark P, Weiner OD, Srinivasan S, Servant G, Bourne HR
Journal:Nat Cell Biol
PubMed ID:12080345
In gradients of external chemo-attractant, mammalian neutrophilic leukocytes (neutrophils) and Dictyostelium discoideum amoebae adopt a polarized morphology and selectively accumulate lipid products of phosphatidylinositol-3-OH kinases (PI(3)Ks), including PtdIns(3,4,5)P(3), at their up-gradient edges; the internal PtdIns(3,4,5)P(3) gradient substantially exceeds that of the external attractant. An accompanying report presents evidence for a ... More
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