NuPAGE™ Bis-Tris Midi Protein Gels, 4 to 12%, 1.0 mm

Los geles de proteína Invitrogen NuPAGE Bis-Tris son geles de poliacrilamida prefundidos que proporcionan una amplia separación de proteínas de peso molecular con alta resolución e integridad de las muestras. Estos geles prefundidos son ideales para aplicaciones en las que la integridad de las proteínas es crucial. A diferencia de los geles tradicionales de Tris-glicina, los geles NuPAGE Bis-Tris tienen un ambiente de pH neutro que minimiza las modificaciones de proteínas. Utilice los geles NuPAGE Bis-Tris para preparar las proteínas para la secuenciación, la espectrometría de masas y cualquier otra técnica donde la integridad de las proteínas resulte crucial.

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.

Features of NuPAGE Bis-Tris gels include:

• Better protein integrity—optimized sample preparation process preserves proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer for optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—store for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation

Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 x 8 cm) or midi (8.7 x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer (Cat. No. NP0007) and NuPAGE Sample Reducing Agent (NP0004). Use NuPAGE Antioxidant (NP0005) in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer (NP0002) to better resolve small proteins or NuPAGE MOPS SDS Running Buffer (NP000102) to resolve medium- to large-size proteins.

NuPAGE Bis-Tris Midi gels can be used with the XCell SureLock Midi Cell gel apparatuses (WR0100) or with the Bio-Rad Criterion Cell using our adapters (WA0999).

For transfer of proteins to a membrane, rapid semi-dry transfer can be done using the Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001). Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell and NuPAGE Transfer buffer (NP00061).