Search
Citations & References (5)
Thermo Scientific™
Sulfo-GMBS (N-γ-maleimidobutyryl-oxysulfosuccinimide ester)
Thermo Scientific Pierce Sulfo-GMBS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends ofRead more
| Catalog Number | Quantity |
|---|---|
| 22324 | 50 mg |
Catalog number 22324
Price (BRL)
***
Quantity:
50 mg
Price (BRL)
***
Thermo Scientific Pierce Sulfo-GMBS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 angstroms).
Features of Sulfo-GMBS:
• Reactive groups: sulfo-NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• Non-cleavable
• Water-soluble (compare to GMBS)
• Has a low potential for illiciting crosslinker immune response, ensuring that the primary response to the antigen-carrier protein conjugate is not diluted by a response against a determinant on the crosslinker
• GMBS and Sulfo-GMBS are reported to be less immunogenic than SMCC
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Related Products
GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)
Features of Sulfo-GMBS:
• Reactive groups: sulfo-NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• Non-cleavable
• Water-soluble (compare to GMBS)
• Has a low potential for illiciting crosslinker immune response, ensuring that the primary response to the antigen-carrier protein conjugate is not diluted by a response against a determinant on the crosslinker
• GMBS and Sulfo-GMBS are reported to be less immunogenic than SMCC
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Related Products
GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityNo
DescriptionSulfo-GMBS
FormPowder
Labeling MethodChemical Labeling
Molecular Weight (g/mol)382.28
PEGylatedNo
Product LinePierce
Quantity50 mg
Reactive MoietyMaleimide, Sulfo-NHS Ester
Shipping ConditionAmbient
SolubilityWater
Spacer Arm Length7.3 Å
Water SolubleYes
Chemical ReactivityAmine-Sulfhydryl
CleavableNo
Crosslinker TypeHeterobifunctional
FormatStandard
Product TypeCrosslinker
SpacerShort (<10 Å)
Unit SizeEach
Contents & Storage
Upon receipt store desiccated at -20°C.
Frequently asked questions (FAQs)
Can you provide the shelf-life for Sulfo-GMBS (N-γ-maleimidobutyryl-oxysulfosuccinimide ester)?
Citations & References (5)
Citations & References
Abstract
Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms.
Journal:Nucleic Acids Res
PubMed ID:11024189
Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides
Ligand-receptor and receptor-receptor interactions act in concert to activate signaling in the Drosophila toll pathway.
Journal:J Biol Chem
PubMed ID:15795223
In Drosophila, the signaling pathway mediated by the Toll receptor is critical for the establishment of embryonic dorso-ventral pattern and for innate immune responses to bacterial and fungal pathogens. Toll is activated by high affinity binding of the cytokine Spätzle, a dimeric ligand of the cystine knot family. In vertebrates,
Characterization of the oligomeric structure of the Ca(2+)-activated Cl- channel Ano1/TMEM16A.
Journal:J Biol Chem
PubMed ID:21056985
Members of the Anoctamin (Ano)/TMEM16A family have recently been identified as essential subunits of the Ca(2+)-activated chloride channel (CaCC). For example, Ano1 is highly expressed in multiple tissues including airway epithelia, where it acts as an apical conduit for transepithelial Cl(-) secretion and helps regulate lung liquid homeostasis and mucus
Studying the protein organization of the postsynaptic density by a novel solid phase- and chemical cross-linking-based technology.
Journal:Mol Cell Proteomics
PubMed ID:16501281
Agarose beads carrying a cleavable, fluorescent, and photoreactive cross-linking reagent on the surface were synthesized and used to selectively pull out the proteins lining the surface of supramolecules. A quantitative comparison of the abundances of various proteins in the sample pulled out by the beads from supramolecules with their original
Rat liver carnitine palmitoyltransferase 1 forms an oligomeric complex within the outer mitochondrial membrane.
Journal:J Biol Chem
PubMed ID:17650509
Carnitine palmitoyltransferase (CPT) 1A catalyzes the rate-limiting step in the transport of long chain acyl-CoAs from cytoplasm to the mitochondrial matrix by converting them to acylcarnitines. Located within the outer mitochondrial membrane, CPT1A activity is inhibited by malonyl-CoA, its allosteric inhibitor. In this study, we investigate for the first time