mirVana™ miRNA Mimic, miR-1 Positive Control
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mirVana™ miRNA Mimic, miR-1 Positive Control

miRNA mimics are small, chemically modified double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules andRead more
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Catalog NumberQuantity
446406450 nmol
44640625 nmol
446406320 nmol
4464065250 nmol
Catalog number 4464064
Price (BRL)
12.077,58
Each
Add to cart
Quantity:
50 nmol
Price (BRL)
12.077,58
Each
Add to cart

miRNA mimics are small, chemically modified double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA functional analysis by up-regulation of miRNA activity.

We have now released the second generation of miRNA mimics, mirVana™ miRNA Mimics, which:

  • Exhibit maximum and consistent effect in vitro at low concentration
  • Offer superior specificity due to unique Star strand modification
  • Target the most updated Sanger content
  • Can be used in vitro and in vivo, offering you consistency throughout your entire research project
  • Are non-toxic

Positive and negative controls are also available for use along with mirVana™ miRNA Mimics in miRNA up-regulation experiments.

mirVana™ miRNA Mimic miR-1 Positive Control

The mirVana™ miRNA Mimic miR-1 Positive Control is designed to mimic mature miR-1 miRNA after delivery to mammalian cells. miR-1 is associated with down-regulation of many genes. In particular, miR-1-mediated down-regulation of Protein Tyrosine Kinase 9 (PTK9) has been shown to occur at the mRNA level. miR-1 Positive Control is intended for use as a positive control for transfection experiments using mirVana™ miRNA Mimics. Monitor activity of miR-1 Positive Control through its gene silencing of PTK9, using real-time reverse transcription-PCR to detect PTK9 mRNA.

First Generation Mimic Controls

Our first-generation miRNA mimics (and controls), Ambion™ Pre-miR™ miRNA Precursors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Mimics. These first-generation mimics are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Mimics and their corresponding controls.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)miRNA & Non-Coding RNA Analysis
Label or DyeUnconjugated
Product LinemirVana
Product TypemiRNA Mimic
Purification MethodHPLC
Quantity50 nmol
Shipping ConditionRoom Temperature
Control TypePositive Control
RNAi TypemiRNA Mimic
Unit SizeEach
Contents & Storage
Store at or below -20°C. Do not store in a frost-free freezer.

Frequently asked questions (FAQs)

How can I quantify endogenous miRNA inactivation by mirVana inhibitors?

Using TaqMan miRNA assays, you can measure the level of miRNA of interest. If the miRNA is bound to the synthetic anti-miRs and/or mirVana inhibitors transfected, the duplex should not be detected, indicating efficient miRNA inactivation. Please note, this only works when total RNA sample isolated is not heat denatured when RT primer is added, as heat denaturation will result in complex dissociation and subsequent detection of microRNA by TaqMan miRNA assay even when cells have been transfected properly. Please see the protocol for the TaqMan MicroRNA Cells-to-CT kit (Cat. No. 4391848) for more details.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How do you introduce mirVana miRNA mimics or inhibitors into cells?

Mimics and inhibitors can be transfected or electroporated into cells using parameters similar to those used for siRNA.

Using mirVana miRNA Mimics- For the following applications, use a cell line that expresses relatively low levels of the mature miRNA of interest.
- Cell density at the time of transfection. In general, 30-70% confluency is recommended.
- Transfection method (traditional or reverse transfection).
- Length of exposure of cells to transfection agent-RNA oligonucleotides complexes.
For many miRNA mimics, maximal activity is achieved after 24 hours, but biological assays may be performed 24-72 hours posttransfection. If you maintain your transfected cells longer than 24 hours, we suggest replacing the existing medium with fresh medium at 24 hours posttransfection, for greater viability of the cells.

Using mirVana miRNA Inhibitors to analyze endogenous miRNA function
For the following applications, use a cell line that expresses relatively high levels of the mature miRNA of interest.
General transfection starting points
mirVana miRNA Mimics and Inhibitors typically work best when transfected at a final concentration of 3-30 nM. Optimization experiments might include a concentration range of 1-100 nM. To increase accuracy and reproducibility when preparing transfection complexes, prepare a dilution of your stock oligonucleotides, and pipet a higher volume of diluted stock.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What is the turnaround time for your mirVana miRNA Mimic and Inhibitors?

The turnaround time varies depending on the level of purification and whether it is a custom order or not. Please see the approximate timetable below:

Standard purification (5 and 20 nmol), 4 business days
HPLC/in vivo ready (250 nmol), 15 business days
Custom mirVana Mimics and Inhibitors, 15 business days

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I would like to create a custom mirVana mimic and inhibitor. Can I do this?

Yes, please provide us with your unpublished miRNA sequence and we can synthesize a corresponding mirVana mimic or inhibitor by clicking on this link (www5.invitrogen.com/custom-genomic-products/tools/mirna/).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Can I get the sequence for the mirVana Mimics and Inhibitors negative control?

Negative control sequences require a confidential disclosure agreement while target sequences require prior proof of purchase for release.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

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