mirVana™ miRNA Inhibitor, Negative Control #1
<i>mir</i>Vana&trade; miRNA Inhibitor, Negative Control #1
Invitrogen™

mirVana™ miRNA Inhibitor, Negative Control #1

mirVana miRNA Inhibitor Negative Control No. 1 is a non-targeting control for miRNA inhibition experiments. It provides a baseline for evaluating effects on gene expression. Extensively tested in human cells, it ensures no impact on known miRNA functions.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
446407850 nmol
44640765 nmol
446407720 nmol
4464079250 nmol
Catalog number 4464078
Price (BRL)
11.676,60
Each
Add to cart
Quantity:
50 nmol
Price (BRL)
11.676,60
Each
Add to cart

mirVana miRNA Inhibitor Negative Control No. 1 is a non-targeting control designed for use in miRNA inhibition experiments. It provides a reliable baseline for evaluating the effects on target gene expression by ensuring minimal off-target effects. Extensively tested in human cell lines and tissues, this control is validated to have no impact on known miRNA functions, making it essential for accurate and consistent gene expression studies. Its properties include high reliability, superior specificity, and versatile use in various experimental setups.

Features of mirVana miRNA Inhibitor Negative Control No. 1 include:

  • Reliability—maximum and consistent effect in vitro at low concentration and low toxicity
  • Potency—better potency than lead competitor's product
  • Superior specificity—the most updated Sanger content
  • Versatile use—can be used in vitro and in vivo, offering you consistency throughout your entire research project

What are mirVana miRNA Inhibitors?

mirVana miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity. Positive and negative controls are also available for use along with mirVana miRNA inhibitors in miRNA down-regulation experiments.

Quality control

Each mirVana miRNA Inhibitor is synthesized and purified in state-of-the-art facilities to meet the highest quality standards. Our rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. We also assess each miRNA Inhibitor by gel electrophoresis to ensure a premium-quality miRNA inhibitor that is purified and ready for use.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)miRNA & Non-Coding RNA Analysis
Label or DyeUnconjugated
Product LinemirVana
Product TypemiRNA Inhibitor
Purification MethodPurified
Quantity50 nmol
Shipping ConditionRoom Temperature
Control TypeNegative Control
RNAi TypemiRNA Inhibitor
Unit SizeEach
Contents & Storage
Store at or below -20°C. Do not store in a frost-free freezer.

Frequently asked questions (FAQs)

How can I quantify endogenous miRNA inactivation by mirVana inhibitors?

Using TaqMan miRNA assays, you can measure the level of miRNA of interest. If the miRNA is bound to the synthetic anti-miRs and/or mirVana inhibitors transfected, the duplex should not be detected, indicating efficient miRNA inactivation. Please note, this only works when total RNA sample isolated is not heat denatured when RT primer is added, as heat denaturation will result in complex dissociation and subsequent detection of microRNA by TaqMan miRNA assay even when cells have been transfected properly. Please see the protocol for the TaqMan MicroRNA Cells-to-CT kit (Cat. No. 4391848) for more details.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How do you introduce mirVana miRNA mimics or inhibitors into cells?

Mimics and inhibitors can be transfected or electroporated into cells using parameters similar to those used for siRNA.

Using mirVana miRNA Mimics- For the following applications, use a cell line that expresses relatively low levels of the mature miRNA of interest.
- Cell density at the time of transfection. In general, 30-70% confluency is recommended.
- Transfection method (traditional or reverse transfection).
- Length of exposure of cells to transfection agent-RNA oligonucleotides complexes.
For many miRNA mimics, maximal activity is achieved after 24 hours, but biological assays may be performed 24-72 hours posttransfection. If you maintain your transfected cells longer than 24 hours, we suggest replacing the existing medium with fresh medium at 24 hours posttransfection, for greater viability of the cells.

Using mirVana miRNA Inhibitors to analyze endogenous miRNA function
For the following applications, use a cell line that expresses relatively high levels of the mature miRNA of interest.
General transfection starting points
mirVana miRNA Mimics and Inhibitors typically work best when transfected at a final concentration of 3-30 nM. Optimization experiments might include a concentration range of 1-100 nM. To increase accuracy and reproducibility when preparing transfection complexes, prepare a dilution of your stock oligonucleotides, and pipet a higher volume of diluted stock.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What is the turnaround time for your mirVana miRNA Mimic and Inhibitors?

The turnaround time varies depending on the level of purification and whether it is a custom order or not. Please see the approximate timetable below:

Standard purification (5 and 20 nmol), 4 business days
HPLC/in vivo ready (250 nmol), 15 business days
Custom mirVana Mimics and Inhibitors, 15 business days

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I would like to create a custom mirVana mimic and inhibitor. Can I do this?

Yes, please provide us with your unpublished miRNA sequence and we can synthesize a corresponding mirVana mimic or inhibitor by clicking on this link (www5.invitrogen.com/custom-genomic-products/tools/mirna/).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Can I get the sequence for the mirVana Mimics and Inhibitors negative control?

Negative control sequences require a confidential disclosure agreement while target sequences require prior proof of purchase for release.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

View all mirVana™ miRNA Inhibitor, Negative Control #1 FAQs