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Description | Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL |
Disposable | Single-use |
Filter Type | Membrane Filter |
Format | Microcentrifuge, Spin Column |
Material (Membrane) | PES |
MWCO | 10 kDa |
Sample Volume | 100 to 500 μL |
Product Line | Pierce™ |
Purification Target | Protein |
Quantity | 25 Concentrators |
Shipping Condition | Room Temperature |
Type | Filter |
Unit Size | 25 concentrators |
Description | Pierce™ Protein Concentrator PES, 10K MWCO, 2-6 mL |
Disposable | Single-use |
Material (Membrane) | PES |
MWCO | 10 kDa |
Sample Volume | 2 to 6 mL |
Product Line | Pierce™ |
Purification Target | Protein |
Quantity | 10 Concentrators |
Shipping Condition | Room Temperature |
Type | Filter |
Unit Size | 10 concentrators |
Description | Pierce™ Protein Concentrator PES, 10K MWCO, 2-6 mL |
Disposable | Single-use |
Material (Membrane) | PES |
MWCO | 10 kDa |
Sample Volume | 2 to 6 mL |
Product Line | Pierce™ |
Purification Target | Protein |
Quantity | 24 Concentrators |
Shipping Condition | Room Temperature |
Type | Filter |
Unit Size | 24 concentrators |
Description | Pierce™ Protein Concentrator PES, 10K MWCO, 5-20 mL |
Disposable | Single-use |
Material (Membrane) | PES |
MWCO | 10 kDa |
Sample Volume | 5 to 20 mL |
Product Line | Pierce™ |
Purification Target | Protein |
Quantity | 10 Concentrators |
Shipping Condition | Room Temperature |
Type | Filter |
Unit Size | 10 concentrators |
Description | Pierce™ Protein Concentrator PES, 10K MWCO, 5-20 mL |
Disposable | Single-use |
Material (Membrane) | PES |
MWCO | 10 kDa |
Sample Volume | 5 to 20 mL |
Product Line | Pierce™ |
Purification Target | Protein |
Quantity | 24 Concentrators |
Shipping Condition | Room Temperature |
Type | Filter |
Unit Size | 24 concentrators |
Description | Pierce™ Protein Concentrator PES, 10K MWCO, 20-100 mL |
Disposable | Single-use |
Material (Membrane) | PES |
MWCO | 10 kDa |
Sample Volume | 20 to 100 mL |
Product Line | Pierce™ |
Purification Target | Protein |
Quantity | 4 Concentrators |
Shipping Condition | Room Temperature |
Type | Filter |
Unit Size | 4 concentrators |
Catalog Number | Specifications | Unit Size | Sample Volume | Quantity | Price (BRL) | Availability | Quantity | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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88513 | 25 concentrators | 100 to 500 μL | 25 Concentrators | Price: 870,40 Your price: | *** | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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88516 | 10 concentrators | 2 to 6 mL | 10 Concentrators | Price: 555,20 Your price: | *** | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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88517 | 24 concentrators | 2 to 6 mL | 24 Concentrators | Price: 1.100,80 Your price: | *** | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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88527 | 10 concentrators | 5 to 20 mL | 10 Concentrators | Price: 1.094,40 Your price: | *** | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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88528 | 24 concentrators | 5 to 20 mL | 24 Concentrators | Contact Us › | *** | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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88535 | 4 concentrators | 20 to 100 mL | 4 Concentrators | Price: 1.260,80 Your price: | *** | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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For protein and other biological samples, such as nucleic acids, with a 10K MWCO, Pierce Protein Concentrators PES offer rapid protein concentration, excellent sample recovery, and instrument compatibility. These centrifugal devices feature a high-flux polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. These concentrators have a MWCO of 10K and can be used for processing sample volumes from 0.5 to 100 mL.
Features of 10K MWCO Protein Concentrators PES include:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 or fewer minutes using the 0.5-mL concentrator and 15 or fewer minutes using the other quantity concentrators
• Excellent protein recovery—typical protein recovery is >90% (when the sample mass is at least two-fold greater than the posted MWCO)
• Instrument-compatibility—a standard microcentrifuge with swinging (except 88513) or fixed-angle rotors can be used; sample can be collected and recovered without invert spinning
• Versatile—easily perform sample concentration, diafiltration, or buffer-exchange of biological samples
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device, enabling visual determination of the retentate volume. The vertical design of the membrane allows high-flux rates, low non-specific binding, and negligible protein aggregation on the membrane, giving reliable and consistent results for processing sample volumes between 0.5 and 100 mL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. For example, when concentrating IgG (MW ∼150 KDa) samples, use the 3K, 10K, 30K, or 50K MWCO concentrators. Final sample concentrations of 10- to 30-fold can be achieved within 10 minutes when using the 0.5 mL concentrators and within 15 minutes when using the other concentrator sizes.
Protein Concentrators PES applications
• Protein concentration of tissue culture media, antiserum, or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after elution of protein from ion-exchange
• Separation of different sized molecules
Pierce Protein Concentrators PES centrifugal devices contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration.
The dead-stop volume for the 0.5 mL, 2–6 mL, 5–20 mL, and 20–100 mL concentrators is approximately 15 μL, 30 μL, 50 μL, and 350 μL, respectively.
To achieve >90% recovery of protein with the 0.5 mL concentrators, the minimum protein sample concentration should be 0.05 mg/mL. For all other concentrators, the minimum protein sample concentration should be 0.1 mg/mL.
Optimal centrifuges and speeds
The 0.5-mL concentrators can be used effectively at a maximum centrifugal force of 15,000 × g, using a fixed angle centrifuge only.
For the 2-6 mL concentrators, swinging-bucket or fixed-angle centrifuge models can be used. Swinging bucket centrifuges capable of generating 2,000–4,000 × g and a maximum centrifugal force of 4,000 × g are recommended. With fixed-angle centrifuges, models capable of generating 5,000–10,000 × g and a maximum centrifugal force of 8,000 × g are recommended.
For the 5-20 mL volume concentrators, swinging-bucket or fixed-angle centrifuge models can be used. Swinging-bucket centrifuges capable of generating 2,000–5,000 × g and a maximum centrifugal force of 4,000 × g are recommended. With fixed-angle centrifuges, models capable of generating 5,000–8,000 × g and a maximum centrifugal force of 6,000 × g are recommended.
For the 20-100 mL concentrators, only swinging-bucket centrifuges capable of generating 1,000–2,000 × g are recommended. Use a maximum centrifugal force of 2,000 × g for the 20–100 mL concentrators. The concentrators are not recommended for use in fixed-angle rotors.
Centrifugal force, temperature and sample volume, concentration and viscosity will affect filtration rate and require optimization of time for each application.