StemPro™ Adipogenesis Differentiation Kit

Citations & References (3)

Gibco™

StemPro™ Adipogenesis Differentiation Kit

For Complete Differentiation of Human Mesenchymal Stem Cells into Fat CellsStemPro™ Adipogenesis Differentiation Kit has been developed for the adipogenicRead more

Catalog Number	Quantity
A1007001	1 Kit

Catalog number A1007001

Price (BRL)

Quantity:

1 Kit

For Complete Differentiation of Human Mesenchymal Stem Cells into Fat Cells

StemPro™ Adipogenesis Differentiation Kit has been developed for the adipogenic differentiation of mesenchymal stem cells (MSCs) in tissue culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the adipogenesis pathway and generate adipocytes. Using StemPro™ Adipogenesis Differentiation Kit in combination with StemPro™ MSC SFM or MesenPRO RS™ Medium provides a standardized culture workflow solution for MSC isolation, expansion and differentiation into lipid vesicle-forming adipocytes.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell TypeStem Cells (Mesenchymal)

Culture EnvironmentCO2

Endotoxin Level≤50 EU/mL, ≤ 50 EU/ml

FormatLiquid

GlucoseHigh Glucose

GlutamineGlutaMAX™-I

HEPES BufferNo HEPES

Inorganic SaltsCalcium, Sodium Phosphate

Phenol Red IndicatorNo Phenol Red

Purity or Quality GradeResearch Grade

Quantity1 Kit

Research CategoryDifferentiation

Serum LevelStandard Serum

Sodium Bicarbonate BufferSodium Bicarbonate

Sodium Pyruvate AdditiveSodium Pyruvate

SpeciesHuman

SupplementsAdipogenesis Supplements

For Use With (Application)Stem Cell Differentiation

Product LineGibco, StemPro

Product TypeAdipogenesis Differentiation Kit

Unit SizeEach

Contents & Storage

This kit is shipped in two parts with separate storage requirements. These components are not sold individually:
• StemPro™ Adipocyte Differentiation Basal Medium (100 ml; store in the dark at 2°C to 8°C)
• StemPro™ Adipogenesis Supplement (10 ml; store in the dark at -5°C to -20°C)

The Basal Medium ships at room temperature, while the Supplement ships on dry ice.

Frequently asked questions (FAQs)

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).

What are mesenchymal stem cells?

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).

Citations & References (3)

Citations & References

Abstract

Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media.

Authors:Oikonomopoulos A, van Deen WK, Manansala AR, Lacey PN, Tomakili TA, Ziman A, Hommes DW,

Journal:

PubMed ID:26564250

'Due to their immunosuppressive properties, mesenchymal stem cells (MSC) have been evaluated for the treatment of immunological diseases. However, the animal-derived growth supplements utilized for MSC manufacturing may lead to clinical complications. Characterization of alternative media formulations is imperative for MSC therapeutic application. Human BMMSC and AdMSC were expanded in ... More

Alginate-Encapsulation for the Improved Hypothermic Preservation of Human Adipose-Derived Stem Cells.

Authors:Swioklo S, Constantinescu A, Connon CJ,

Journal:Stem Cells Transl Med

PubMed ID:26826163

: Despite considerable progress within the cell therapy industry, unmet bioprocessing and logistical challenges associated with the storage and distribution of cells between sites of manufacture and the clinic exist. We examined whether hypothermic (4°C-23°C) preservation of human adipose-derived stem cells could be improved through their encapsulation in 1.2% calcium ... More

Human Synovial MSC Good Manufacturing Practices for Articular Cartilage Regeneration.

Authors:Fernandes TL, Kimura HA, Pinheiro CCG, Shimomura K, Nakamura N, Ferreira JRM, Gomoll AH, Hernandez AJ, Bueno DF,

Journal:Tissue Eng Part C Methods

PubMed ID:30412046

cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite Autologous Chondrocyte Implantation (ACI) versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal ... More