SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays
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Invitrogen™

SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays

The SuperScript® One-Cycle cDNA Kit for use with Affymetrix® One-Cycle Assays conveniently contains all of the reagents required for double-strandedRead more
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Catalog NumberQuantity
A1075203030 Reactions
Catalog number A10752030
Price (BRL)
27.035,56
Each
Add to cart
Quantity:
30 Reactions
Price (BRL)
27.035,56
Each
Add to cart
The SuperScript® One-Cycle cDNA Kit for use with Affymetrix® One-Cycle Assays conveniently contains all of the reagents required for double-stranded cDNA synthesis within the Affymetrix GeneChip® workflow. The kit includes Invitrogen’s proprietary RNase H reduced activity SuperScript® II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix’ protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)For generating double-stranded cDNA
For Use With (Equipment)Affymetrix GeneChip™
Includes Label or DyeNo
Labeling MethodDirect Labeling
Product LineSuperScript
Product TypecDNA Kit
Quantity30 Reactions
Shipping ConditionDry Ice
Detection MethodBiotin-based
Final Product TypecDNA (Double-stranded)
FormatKit
Labeling TargetcDNA
Label or DyeBiotin
Sample TypePoly(A+) RNA, Total RNA
Unit SizeEach
Contents & Storage
50 μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript™ II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water
All components are guaranteed stable for 6 months when properly stored.