QuantStudio™ 3 Real-Time PCR System, 96-well, 0.2 mL, laptop
QuantStudio™ 3 Real-Time PCR System, 96-well, 0.2 mL, laptop
Applied Biosystems™

QuantStudio™ 3 Real-Time PCR System, 96-well, 0.2 mL, laptop

The Applied Biosystems QuantStudio 3 Real-Time PCR System is designed for users who need an affordable, easy-to-use real-time PCR systemRead more
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Catalog NumberQuantity
A285671 system
Catalog number A28567
Price (BRL)
-
Quantity:
1 system
The Applied Biosystems QuantStudio 3 Real-Time PCR System is designed for users who need an affordable, easy-to-use real-time PCR system that doesn't compromise performance and quality. The simplified Design and Analysis software is ideal for both first-time and experienced users. When connected to iConnect, Thermo Fisher's cloud-based platform, the QuantStudio 3 system provides access to your data wherever and whenever you want. Using proven OptiFlex technology (featuring four coupled channels and white LED) and featuring three independent Veriflex temperature zones, the QuantStudio 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications.

Quantstudio 3/5 Firmware and Design & Analysis Software >

Features of the QuantStudio 3 system include:

• Simplified instrument software with interactive touchscreen for greater ease of use
• Proven OptiFlex technology (four coupled channels with white LED) for trusted results
• Secure cloud-based software option provides increased accessibility and storage options
• User account management, locked workflow, and Pause features for greater control of experiments
• 96-well 0.2 mL format or 96-well 0.1 mL format

iConnect
iConnect provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within iConnect are web browser-based software tools that are flexible, fast and easy to use.

Easy to use
The QuantStudio 3 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software options:

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows 10 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology, which features four coupled excitation and emission filter channels with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Display TypeTouchscreen
For Use With (Equipment)QuantStudio™
Format96-well Plate
Product LineQuantStudio
Quantity1 system
Shipping ConditionRoom Temperature
Volume (Metric) Thermal Block Sample0.2 ml
Block FormatNon-interchangeable
Unit SizeEach
Contents & Storage
Laptop Computer

Frequently asked questions (FAQs)

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Is the black frame removable from the QuantStudio 3 and 5 Real-Time PCR Systems 96 well, 0.2 ml block?

We do not recommend physically removing the black frame. It is necessary for the plates to fit properly and should stay on the instrument. If it has been accidently removed or lost, please contact ICS to replace the spare part at (800) 9550-6288 options 3-2 or email technicalhardwaresupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Can I import a standard curve for my real-time PCR data into the QuantStudio Design & Analysis Software (v1.x)?

If you are analyzing results from the QuantStudio 1 Real-Time PCR System, QuantStudio 3 Real-Time PCR System, or QuantStudio 5 Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Design & Analysis Software (v1.x) following the instructions below:
1. Open the data file in the QuantStudio Design & Analysis Software (v1.x).
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply.

Can you provide some tips for setting up an absolute quantification/standard curve on the QuantStudio 3 or 5 Real-Time PCR instrument?

Please review this guided tutorial that explains how to set up an Absolute Quantification/Standard Curve experiment run on your QuantStudio 3 or 5 Real-Time PCR instrument: https://www.brainshark.com/thermofisher/QS35AQSetupTutorial

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Citations & References (3791)

Citations & References
Abstract
Immune-Enhancing Effects of Crude Polysaccharides from Korean Ginseng Berries on Spleens of Mice with Cyclophosphamide-Induced Immunosuppression.
Authors:Nam JH,Choi J,Monmai C,Rod-In W,Jang AY,You S,Park WJ
Journal:Journal of microbiology and biotechnology
PubMed ID:34949747
Panax ginseng C. A. Meyer is well known as traditional herbal medicine, and ginseng berries are known to exhibit potential immune-enhancing functions. However, little is known about the in vivo immunomodulatory activity of Korean ginseng berries. In this study, crude Korean ginseng berries polysaccharides (GBP) were isolated and their immunomodulatory ... More
Excessive manganese alters serum biochemical indices, induces histopathological alterations, and activates apoptosis in liver and cerebrum of Jianzhou Da'er goat (Capra hircus).
Authors:Xiang Y,Wang L,Wei Y,Zhang H,Emu Q
Journal:Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
PubMed ID:34752896
Identification of osteopontin as a urinary biomarker for autosomal dominant polycystic kidney disease progression.
Authors:Kim H,Sung J,Bae JY,Lee P,Oh YK,Kim H
Journal:Kidney research and clinical practice
PubMed ID:35791741
BACKGROUND: Autosomal dominant polycystic kidney disease (ADPKD), one of the most common human monogenic diseases, is characterized by the presence of numerous fluid-filled renal cysts and is a leading cause of end-stage renal disease (ESRD). Urinary biomarkers may be useful for predicting the variable course of ADPKD progression from cyst ... More
Evidence for crosstalk between the aryl hydrocarbon receptor and the translocator protein in mouse lung epithelial cells.
Authors:Steidemann MM,Liu J,Bayes K,Castro LP,Ferguson-Miller S,LaPres JJ
Journal:Experimental cell research
PubMed ID:37172753
Cellular homeostasis requires the use of multiple environmental sensors that can respond to a variety of endogenous and exogenous compounds. The aryl hydrocarbon receptor (AHR) is classically known as a transcription factor that induces drug metabolizing enzymes when bound to toxicants such as 2,3,7,8-tetrachlorodibenzo-ρ-dioxin (TCDD). The receptor has a growing ... More
AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphology.
Authors:Tilokani L,Russell FM,Hamilton S,Virga DM,Segawa M,Paupe V,Gruszczyk AV,Protasoni M,Tabara LC,Johnson M,Anand H,Murphy MP,Hardie DG,Polleux F,Prudent J
Journal:Science advances
PubMed ID:36367943
Mitochondria are dynamic organelles that undergo membrane remodeling events in response to metabolic alterations to generate an adequate mitochondrial network. Here, we investigated the function of mitochondrial fission regulator 1-like protein (MTFR1L), an uncharacterized protein that has been identified in phosphoproteomic screens as a potential AMP-activated protein kinase (AMPK) substrate. ... More