StemFlex™ Medium

Citations & References (10)

Gibco™

StemFlex™ Medium

StemFlex Medium enables long term feeder-free culture of pluripotent stem cells (PSCs) and is optimized to support challenging applications, including single-cell passaging, gene editing, and reprogramming.

Catalog Number	Quantity
A3349401	500 mL

Catalog number A3349401

Price (BRL)

Quantity:

500 mL

StemFlex Medium's unique formulation supports robust expansion of PSCs while offering the convenience of a flexible feeding schedule (including weekend-free options) and the ability to choose the matrix and passaging reagent that best suits specific applications. StemFlex Medium enables the long term feeder-free culture of PSCs without karyotypic abnormalities and maintains the cells' ability to differentiate into all three germ layers.

StemFlex Medium:

Delivers superior results in challenging applications
Offers flexibility for PSC culture conditions and feeding schedules
Provides exceptional value and performance

Superior results

StemFlex Medium is formulated to deliver superior outcomes in challenging applications that stress PSC cultures, such as reprogramming, single cell passaging, and gene editing. It provides superior cell survival compared to a leading competitor (mTeSR1), allowing for greater success in the most relevant cell types.

Flexibility

StemFlex Medium allows for unprecedented three-way usage flexibility for PSC culture conditions: the ability to select an optimal feeding schedule, including a weekend-free option; a choice of matrices, depending upon the needs of the workflow; and the option to select the passaging reagent for the level of cell dissociation required.

Exceptional value and performance

StemFlex Medium is provided in a convenient two-component kit (450 mL basal medium & 50 mL supplement) and, when used with Geltrex Flex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix, provides a cost-effective, robust system for superior feeder-free culture of human PSCs. StemFlex Medium's ability to enable weekend-free culture of PSCs provides unmatched value among the robust feeder-fee PSC media on the market today.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell LineEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)

Cell TypeEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)

ConcentrationStemFlex Basal Medium (1X); StemFlex Supplement (10X)

Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485

Product LineStemFlex

Product TypeStem Cell Media

Quantity500 mL

Shelf Life12 Months

Shipping ConditionSupplement ships on dry ice, basal can ship at RT

ClassificationAnimal Origin-free

Culture TypeAdherent Cell Culture, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)

FormLiquid

Serum LevelSerum-free

SterilitySterile-filtered

Sterilization MethodSterile-filtered

With AdditivesPhenol Red

Unit SizeEach

Contents & Storage

• 450 mL Basal Medium, store at 2–8°C and protect from light
• 50 mL Supplement, store at -5 to -20°C and protect from light

Frequently asked questions (FAQs)

You recommend Geltrex matrix as the matrix for routine culture of StemFlex Medium. Can I also use Vitronectin or rhLaminin-521?

Yes. We recommend following the coating instructions for Vitronectin or rhLaminin-521 and using the culture and passaging recommendations in the StemFlex protocol.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for human fibroblasts?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of both neonatal and adult human dermal fibroblasts. For fibroblasts, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 16-20). On Day 7, you may use rhVTN-N (Cat. No. A400457 or A400458), Geltrex Flex matrix (Cat. No. A4000046801, A4000046802, or A4000046803), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, we recommend that you feed reprogrammed fibroblasts every-other-day with StemFlex Medium.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for blood cells?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of CD34+ blood cells. For CD34+ cells, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 39-44). On Day 3, you can utilize rhVTN-N (Cat. No. A400457 or A400458), Geltrex Flex matrix (Cat. No. A4000046801, A4000046802, or A4000046803), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, reprogrammed CD34+ cells should be fed every-other-day with StemFlex Medium.

What is the glucose content of the StemFlex Medium (Cat. No. A3349401)?

The StemFlex Medium (Cat. No. A3349401) includes 3.151 g/L (~17.5 mM) of D-glucose (dextrose) in the Basal Medium. The StemFlex Medium Supplement does not include glucose.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What media do you recommend for standard single-cell cloning of iPSCs?

We recommend StemFlex Medium (Cat. No. A3349401) or Essential 8 (Cat. No. A1517001) for standard single-cell iPSC cloning in the presence of RevitaCell or ROCK inhibitor. StemScale PSC Suspension Medium (Cat. No. A4965001) is not recommended for iPSC cloning.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all StemFlex™ Medium FAQs

Citations & References (10)

Citations & References

Abstract

Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media.

Authors:Daniszewski M, Nguyen Q, Chy HS, Singh V, Crombie DE, Kulkarni T, Liang HH, Sivakumaran P, Lidgerwood GE, Hernández D, Conquest A, Rooney LA, Chevalier S, Andersen SB, Senabouth A, Vickers JC, Mackey DA, Craig JE, Laslett AL, Hewitt AW, Powell JE, Pébay A

Journal:iScience

PubMed ID:30267684

'We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells ... More

Improving single-cell cloning workflow for gene editing in human pluripotent stem cells.

Authors:Chen YH, Pruett-Miller SM

Journal:Stem Cell Res

PubMed ID:30099335

'The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens. Unfortunately, the procedures for genome editing of hPSCs often subject cells to harsh conditions that compromise viability: a major problem that is compounded by ... More

Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing.

Authors:Weisheit I, Kroeger JA, Malik R, Klimmt J, Crusius D, Dannert A, Dichgans M, Paquet D

Journal:Cell Rep

PubMed ID:32460021

'CRISPR genome editing is a promising tool for translational research but can cause undesired editing outcomes, both on target at the edited locus and off target at other genomic loci. Here, we investigate the occurrence of deleterious on-target effects (OnTEs) in human stem cells after insertion of disease-related mutations by ... More

Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells.

Authors:Chen SW, Himeno M, Koui Y, Sugiyama M, Nishitsuji H, Mizokami M, Shimotohno K, Miyajima A, Kido T

Journal:Sci Rep

PubMed ID:32873852

'Hepatocytes derived from human iPSCs are useful to study hepatitis B virus (HBV) infection, however infection efficiency is rather poor. In order to improve the efficiency of HBV infection to iPSC-derived hepatocytes, we set a co-culture of hepatocytes with liver non-parenchymal cells and found that liver sinusoidal endothelial cells (LSECs) ... More

A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons.

Authors:Sridharan B, Hubbs C, Llamosas N, Kilinc M, Singhera FU, Willems E, Piper DR, Scampavia L, Rumbaugh G, Spicer TP

Journal:Sci Rep

PubMed ID:31227747

'Neurons created from human induced pluripotent stem cells (hiPSCs) provide the capability of identifying biological mechanisms that underlie brain disorders. IPSC-derived human neurons, or iNs, hold promise for advancing precision medicine through drug screening, though it remains unclear to what extent iNs can support early-stage drug discovery efforts in industrial-scale ... More

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