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Human Epidermal Melanocytes, neonatal, moderately pigmented donor, (HEMn-MP)

Primary human epidermal melanocytes isolated from neonatal foreskin, cryopreserved at the end of the secondary culture. These cells are isolated from moderately pigmented tissue.
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Catalog NumberQuantity
C1025C1 vial
Catalog number C1025C
Price (BRL)
-
Quantity:
1 vial

Primary human epidermal melanocytes isolated from neonatal foreskin, cryopreserved at the end of the secondary culture. These cells are isolated from moderately pigmented tissue.

  • Viability at least 70%
  • ≥500,000 viable cells/vial
  • Capable of at least 12 population doublings

Tested for

  • Mycoplasma: Not Detected
  • Hepatitis B: Not Detected
  • Hepatitis C: Not Detected
  • HIV-1: Not Detected
  • Bacteria, yeast, and other fungi: Not Detected

For Research Use Only. Not for use in diagnostic procedures.

Specifications
Donor AttributesModerately Pigmented
Donor SourceSingle Donor
No. of Cells≥500,000
Product LineCascade Biologics
Product TypeHuman Epidermal Melanocyte
Quantity1 vial
Tested ForHepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria and Fungi), Mycoplasma, Growth Performance Assay, HIV-1
AgeNewborn (14 days or less)
Cell TypeMelanocyte Cells
Culture TypeAdherent Cell Culture
FormCryopreserved
SpeciesHuman
Unit SizeEach
Contents & Storage
Liquid nitrogen temperature

Frequently asked questions (FAQs)

Do you have ethnicity information for your human epidermal melanocyte donor lines?

We do provide age/sex/pigmentation/ethnicity on the Certificate of Analysis (COA).

How should melanocytes be frozen?

Do not use Synth-a-Freeze Cryopreservation Medium to cryopreserve melanocytes. We recommend using DMEM with 10% FBS and 10% DMSO, or the Recovery Cell Culture Freezing Medium.

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

View all Human Epidermal Melanocytes, neonatal, moderately pigmented donor, (HEMn-MP) FAQs