FluoCells™ Prepared Slide #1 (BPAE cells with MitoTracker™ Red CMXRos, Alexa Fluor™ 488 Phalloidin, and DAPI)

Contains fixed, multi-labeled, cell preparations for observation by fluorescence microscopy validated for immunofluorescence

Catalog Number	Quantity
F36924	1 Ea.

Catalog number F36924

Price (BRL)

2.421,94

Quantity:

1 Ea.

Price (BRL)

2.421,94

FluoCells™ prepared microscope slides contain fixed, multi-labeled cell preparations for observation by fluorescence microscopy. FluoCells™ Prepared Slide #1 contains bovine pulmonary artery endothelial cells (BPAEC). MitoTracker™ Red CMXRos was used to stain the mitochondria in the live cells, with accumulation dependent upon membrane potential. Following fixation and permeabilization, F-actin was stained with Alexa Fluor™ 488 phalloidin, and the nuclei were counterstained with the blue-fluorescent DNA stain DAPI.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)Immunofluorescence

Quantity1 Ea.

Shipping ConditionRoom Temperature

Product LineAlexa Fluor FluoCells MitoTracker

TypePrepared Microscope Slide

Unit SizeEach

Contents & Storage

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

Do you have any slides of pre-labeled cells or tissues that I can use to demonstrate with?

Some of the most popular are the FluoCells demonstration slides, which have cells or tissue sections that are labeled with three different dyes. FluoCells 1 and 2 have cultured cells, while FluoCells 3 is a tissue section (mouse kidney). Slides with fluorescent beads are also available.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (2)

Citations & References

Abstract

Quality assessment of confocal microscopy slide based systems: performance.

Authors:Zucker RM

Journal:Cytometry A

PubMed ID:16807897

'BACKGROUND: All fluorescence slide-based cytometry detections systems basically include the following components: (1) an excitation light source, (2) intermediate optics, and (3) a detection device consisting of a CCD camera or a PMT. The optical principles employed is slide-based systems are similar to those of confocal microscopes (CLSM). METHODS: The ... More

Quality assessment of confocal microscopy slide-based systems: instability.

Authors:Zucker RM

Journal:Cytometry A

PubMed ID:16807898

'BACKGROUND: All slide-based fluorescence cytometry detections systems basically include an excitation light source, intermediate optics, and a detection device (CCD or PMT). Occasionally, this equipment becomes unstable, generating unreliable and inferior data. METHODS: A number of tests have been devised to evaluate equipment performance and instability. The following four instability ... More