Streptavidin, β-galactosidase conjugate

The streptavidin, ß-galactosidase conjugate can be used to detect biotin in a signal amplification scheme in conjunction with chromogenic substratesRead more

Catalog Number	Quantity
S931 also known as S-931	1 mg

Catalog number S931

also known as S-931

Price (BRL)

Quantity:

The streptavidin, ß-galactosidase conjugate can be used to detect biotin in a signal amplification scheme in conjunction with chromogenic substrates or fluorogenic substrates.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product TypeStreptavidin Conjugate (fluorescent)

Quantity1 mg

Shipping ConditionWet Ice

Conjugatebeta-Galactosidase

FormSolid

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -30°C).

Citations & References (5)

Citations & References

Abstract

Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.

Authors:Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ

Journal:J Immunol Methods

PubMed ID:2558138

'A novel signal amplification method, catalyzed reporter deposition (CARD), and its application to immunoassays is described. The method involves utilizing an analyte-dependent reporter enzyme (ADRE) to catalyze the deposition of additional reporter on the surface in a solid-phase immunoassay. In the examples described, deposition of reporter is facilitated by using ... More

Fluorescence probe studies of the state of tropomyosin in reconstituted muscle thin filaments.

Authors:Ishii Y, Lehrer SS

Journal:Biochemistry

PubMed ID:3663633

'The monomer fluorescence of N-(1-pyrenyl)maleimide-labeled tropomyosin bound to F-actin (PTm-actin) increases when myosin subfragment 1 (S1) binds to actin and is half complete when only approximately 1 S1 is bound to 7 actin subunits [Ishii, Y., & Lehrer, S. S. (1985) Biochemistry 24, 6631-6638]. Similar studies of the binding of ... More

Fluorescence sandwich enzyme-linked immunosorbent assay for detecting human interleukin-2 receptors.

Authors:Honda M, Nagao S, Yamamoto N, Tanaka Y, Tozawa H, Tokunaga T

Journal:J Immunol Methods

PubMed ID:2453583

A very sensitive fluorescence sandwich enzyme-linked immunosorbent assay (FS-ELISA) for the detection of soluble and cell-associated human interleukin-2 receptors (IL-2R) has been developed. For use in this assay system, two anti-human IL-2R monoclonal antibodies, H 48 and biotinylated HA 26, were selected from six monoclonal antibodies directed against different epitopes ... More

Measurement of antigen-specific mouse IgE by a fluorometric reverse (IgE-capture) ELISA.

Authors:Sakaguchi M, Inouye S, Miyazawa H, Tamura S

Journal:J Immunol Methods

PubMed ID:2783445

A reverse, or IgE-capture, enzyme-linked immunosorbent assay (ELISA) for measuring ovalbumin-specific IgE antibody in the serum of immunized mice has been developed. Microplate wells were first coated with a commercial anti-mouse IgE rat monoclonal antibody, and then incubated with two-fold serial dilutions of test sera with 10% normal mouse serum ... More

TNF-alpha pretreatment prevents subsequent activation of cultured brain cells with TNF-alpha and hypoxia via ceramide.

Authors:Ginis I, Schweizer U, Brenner M, Liu J, Azzam N, Spatz M, Hallenbeck JM

Journal:Am J Physiol

PubMed ID:10329967

We have developed a cellular model in which cultured astrocytes and brain capillary endothelial cells preconditioned with tumor necrosis factor-alpha (TNF-alpha) fail to upregulate intercellular adhesion molecule-1 (ICAM-1) protein (80% inhibition) and mRNA (30% inhibition) when challenged with TNF-alpha or exposed to hypoxia. Inasmuch as ceramide is known to mediate ... More