For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferPBS Buffer
Concentration1 mg/mL
Detection MethodFluorescence
Excitation/Emission488/767
Flow Cytometer Laser Lines488, 488 or 561, 561
For Use With (Equipment)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
Label or DyePE-Cy7
Product TypeStreptavidin Conjugate (Fluorescent)
Quantity0.5 mL
Shipping ConditionWet Ice
ConjugatePE-Cy7
FormSolution
Product LineCy
Unit SizeEach
Contents & Storage
Contains 1 vial of streptavidin,PE-Cy7 conjugate.
Store in refrigerator (2–8°C).
Citations & References (1)
Citations & References
Abstract
Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen.
Authors:Smith K, Garman L, Wrammert J, Zheng NY, Capra JD, Ahmed R, Wilson PC,
Journal:Nat Protoc
PubMed ID:19247287
We describe herein a protocol for the production of antigen-specific human monoclonal antibodies (hmAbs). Antibody-secreting cells (ASCs) are isolated from whole blood collected 7 d after vaccination and sorted by flow cytometry into single cell plates. The antibody genes of the ASCs are then amplified by RT-PCR and nested PCR, ... More