Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System
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Invitrogen™

Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System

Alternative Product: Try TaqMan Fast Virus 1-Step Master Mix, our high performance, 1-step RT-qPCR master mix. With TaqMan Fast VirusLeia mais
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Número do catálogoNo. of Reactions
11731023500 Reactions
11731015100 Reactions
Número do catálogo 11731023
Preço (BRL)
24.998,75
Each
Adicionar ao carrinho
No. of Reactions:
500 Reactions
Preço (BRL)
24.998,75
Each
Adicionar ao carrinho
Alternative Product: Try TaqMan Fast Virus 1-Step Master Mix, our high performance, 1-step RT-qPCR master mix. With TaqMan Fast Virus 1-Step Master Mix, we’ve improved on the best features of the Platinum Quantitative RT-PCR ThermoScript One-Step System and added additional convenience and performance to your 1-step gene expression analysis and RNA virus detection workflows.
Platinum Quantitative RT-PCR ThermoScript One-Step System is for real-time quantitation of RNA molecules from total or poly(A)+ RNA in a single step.

This kit provides:
• Platinum™ Taq DNA Polymerase
• ThermoScript RT polymerase
• A vial of 50 mM MgSO4

For Research Use Only. Not for use in diagnostic procedures.
Especificações
No. of Reactions500 Reactions
PolymeraseTaq DNA Polymerase
Product LinePlatinum, ThermoScript
Product TypeRT-PCR One-Step System
Quantity500 reactions
Sample TypeRNA
Shipping ConditionDry Ice
Sufficient For500 Reactions
Detection MethodPrimer-probe
PCR Method1-step RT-qPCR
Reaction SpeedStandard
Unit SizeEach
Conteúdo e armazenamento
Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System contains ThermoScript™ RT/Platinum™ Taq mix, and 2X ThermoScript™ reaction mix (a buffer containing 0.4 mM of each dNTP, 6 mM MgSO4 ). A vial of 50 mM MgSO4 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System, 500 reactions FAQs