SuperScript™ VILO™ cDNA Synthesis Kit
SuperScript™ VILO™ cDNA Synthesis Kit
Citações e referências (2)
Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit isLeia mais
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Número do catálogoNo. of Reactions
11754250250 Reactions
1175405050 Reactions
Número do catálogo 11754250
Preço (BRL)
22.466,81
Each
Adicionar ao carrinho
No. of Reactions:
250 Reactions
Request bulk or custom format
Preço (BRL)
22.466,81
Each
Adicionar ao carrinho

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme.
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

For Research Use Only. Not for use in diagnostic procedures.
Especificações
Final Product TypeFirst-Strand cDNA
FormatKit
No. of Reactions250 Reactions
Optimal Reaction Temperature42°C
Quantity250 reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Reverse TranscriptaseSuperScript III
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Size (Final Product)Up to 10 kb
Starting MaterialRNA
TechniqueReverse Transcription
Detection MethodqPCR
For Use With (Application)Real Time PCR (qPCR)
GC-Rich PCR PerformanceHigh
Reaction Speed60 min.
Unit SizeEach
Conteúdo e armazenamento

• 5X VILO Reaction Mix, 1,000 μL
• 10X SuperScript III Enzyme Blend, 500 μL

Store at –5 to –30°C.

Frequently asked questions (FAQs)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

What is the difference between SuperScript III RT and the RT in the SuperScript VILO kit?

The SuperScript VILO cDNA Synthesis Kit contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript

The SuperScript VILO cDNA Synthesis Kit (Cat. No. 11754050) contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript VILO kit is active at 42 degrees C due to the helper proteins.

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

Citações e referências (2)

Citações e referências
Abstract
T cells modulate Epstein-Barr virus latency phenotypes during infection of humanized mice.
Authors:Heuts F, Rottenberg ME, Salamon D, Rasul E, Adori M, Klein G, Klein E, Nagy N,
Journal:
PubMed ID:24390326
Human B cells, the main target of Epstein-Barr virus (EBV), can display several types of latent viral protein expression, denoted 0, I, IIa, IIb, or III. Of these, only type III expression induces proliferation of cells in vitro. These latency types are present at specific stages of infection and are ... More
Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.
Authors:Hulsmans M, Van Dooren E, Mathieu C, Holvoet P,
Journal:PLoS One
PubMed ID:22393448
Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation ... More