Gateway® LR Clonase® enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate your expression clone. We offer different formats of LR Clonase enzyme mixtures, depending on your application and desired format. LR Clonase II Plus enzyme mix is the latest version that offers the highest recombination efficiency available(Table 1), and is specifically optimized for both single and multiple fragment cloning (Table 1). LR Clonase II Plus enzyme mix is provided in an optimized single mix of enzyme and reaction buffer, ensuring enzyme stability and easy of use with few pippetting steps for both single and multiple fragment cloning. Our LR Clonase II enzyme mix is also available in a single mix format, while the original LR Clonase® enzyme is the enzyme and buffer in separate tubes