LR Clonase II for BaculoDirect™ Kits
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Invitrogen™

LR Clonase II for BaculoDirect™ Kits

The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. TheLeia mais
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Número do catálogoQuantity
1179102310 reactions
Número do catálogo 11791023
Preço (BRL)
2.742,96
Each
Adicionar ao carrinho
Quantity:
10 reactions
Preço (BRL)
2.742,96
Each
Adicionar ao carrinho
The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway® Technology with baculovirus expression makes the BaculoDirect™ System the fastest and easiest method for production of recombinant baculovirus.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Product TypeLR Clonase II
Quantity10 reactions
Shipping ConditionDry Ice
EnzymeLR Clonase
Product LineBaculoDirect, Clonase, Gateway
Unit SizeEach
Conteúdo e armazenamento
Store LR Clonase™ mix at -80°C. All components are guaranteed stable for 6 months if stored properly.

Frequently asked questions (FAQs)

Can I use E. coli containing an F' plasmid, such as Top 10F', to propagate a vector with the ccdB gene?

While the F' plasmid does contain the ccdA gene that can inhibit or reduce the toxicity of the ccdB gene product, the ccdA expression level is likely to be too low, or inhibition may not be complete, and the bacteria would still be exposed to the ccdB gene product and thus not grow. Therefore, bacterial strains containing the F' plasmid are not recommended as hosts for propagation of ccdB containing vectors.

For propagation of Gateway vectors containing ccdB, we recommend the One Shot ccdB Survival 2 T1R Competent Cells (A10460), which were specifically designed for that purpose. However, please note that these cells are not validated for propagation of other ccdB-containing vectors like the older pZErO plasmids, and in most cases they are not expected to work due to very high levels of ccdB protein expressed in those vectors.