Platinum™ PCR SuperMix High Fidelity
Platinum™ PCR SuperMix High Fidelity
Citações e referências (2)
Invitrogen™

Platinum™ PCR SuperMix High Fidelity

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
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Número do catálogoNo. of Reactions
125320245000 Reactions
12532016100 Reactions
Número do catálogo 12532024
Preço (BRL)
96.627,88
Each
Adicionar ao carrinho
No. of Reactions:
5000 Reactions
Request bulk or custom format
Preço (BRL)
96.627,88
Each
Adicionar ao carrinho
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Designed for high-specificity, high-fidelity DNA amplification, Platinum PCR SuperMix reduces reaction set-up time and only requires the addition of template and primer.

High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic hot-start PCR.

Benefits of Platinum PCR SuperMix, High Fidelity

  • Six-times higher fidelity than Taq DNA polymerase
  • Amplifies fragments up to 15 kb
  • Minimizes PCR optimization
  • Room-temperature reaction assembly

Applications

  • High-fidelity PCR
  • Cloning
  • Mutagenesis

Notes

  • Platinum PCR SuperMix, High Fidelity, produces products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs.
  • Hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Fidelity (vs. Taq)6X
Hot StartBuilt-In Hot Start
No. of Reactions5000 Reactions
OverhangMixed
PolymerasePlatinum Taq DNA Polymerase High Fidelity
Quantity5,000 reactions
Reaction FormatSuperMix or Master Mix
Shipping ConditionWet or Dry Ice
Size (Final Product)15 kb or less
Concentration1.1X
For Use With (Application)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Conteúdo e armazenamento
Platinum PCR SuperMix High Fidelity (4 x 56.25 mL)

Store at -20°C in a non-frost-free freezer.

Frequently asked questions (FAQs)

What is the difference between Platinum technology and AccuPrime technology?

With Platinum technology, anti-DNA polymerase antibodies bind to the enzyme until the denaturing step at 94 degrees C, when the antibodies degrade. The polymerase is now active and primer extension can occur. AccuPrime Taq combines Platinum Taq (Taq + Platinum antibodies) with proprietary thermostable AccuPrime accessory proteins. The 10X reaction buffer contains the accessory proteins which enhance specific primer-template hybridization during each cycle of PCR.

Can I use MgCl2 instead of MgSO4 with Platinum Taq High Fidelity enzyme?

We strongly suggest using MgSO4. While MgCl2 may work in some cases, MgSO4 usually produces more robust and reproducible products, as sulfate is the best anion found for the Platinum Taq High Fidelity enzyme.

If I do PCR using the Platinum PCR SuperMix High Fidelity, can I use DNA-RNA chimeric oligos such as 5'-AGGCTTggau (lower case letters for RNA bases)?

PCR cannot be performed with an RNA oligo. RNA is not heat stable so the RNA part of the oligo will be degraded. In the presence of Mg++ (PCR buffer contains usually 1-2 mM Mg++) the rate of degradation is much faster.

Citações e referências (2)

Citações e referências
Abstract
Identifying genes of agronomic importance in maize by screening microsatellites for evidence of selection during domestication.
Authors: Vigouroux Y; McMullen M; Hittinger C T; Houchins K; Schulz L; Kresovich S; Matsuoka Y; Doebley J;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12105270
'Crop species experienced strong selective pressure directed at genes controlling traits of agronomic importance during their domestication and subsequent episodes of selective breeding. Consequently, these genes are expected to exhibit the signature of selection. We screened 501 maize genes for the signature of selection using microsatellites or simple sequence repeats ... More
Pattern of diversity in the genomic region near the maize domestication gene tb1.
Authors:Clark RM, Linton E, Messing J, Doebley JF,
Journal:Proc Natl Acad Sci U S A
PubMed ID:14701910
Domesticated maize and its wild ancestor (teosinte) differ strikingly in morphology and afford an opportunity to examine the connection between strong selection and diversity in a major crop species. The tb1 gene largely controls the increase in apical dominance in maize relative to teosinte, and a region of the tb1 ... More