Engineered specifically for culture of human mammary epithelial cells, HuMEC Medium is a serum-free formulation with frozen supplements containing growth factor and other components. This translates to greater experimental validity, more reproducible results, and reduction of lot-to-lot variability and aberrations that can occur with the addition of serum. Human mammary epithelial cells cultured in HuMEC Medium have gene expression profiles consistent with normal mammary epithelial cells, as tested on a cDNA microarray.

Applications:
HuMEC Medium is optimized specifically for the growth of human mammary epithelial cells, and has been performance tested in a growth assay using normal human mammary epithelial cells. HuMEC Medium promotes superior growth of normal human mammary epithelial cells, making it ideal for researchers in breast cancer, drug discovery, and other aspects of breast biology.

Contents and Storage:
HuMEC Ready Medium consists of HuMEC Basal Serum-Free Medium (12753018) supplemented with HuMEC Supplement Kit (12755013). The HuMEC Supplement Kit includes 5 ml of a supplement mix containing epidermal growth factor, hydrocortisone, isoproterenol, transferrin, and insulin, and 25 mg of bovine pituitary extract. Store HuMEC Ready Medium at -5°C to -20°C. Store the supplement mix at -5°C to -20°C.