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Gibco™

Fetal Bovine Serum, Mesenchymal Cell Qualified, US Origin

Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust ofLeia mais
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Número do catálogoQuantity
12763025
conhecido também como 12763-025
500 mL
Número do catálogo 12763025
conhecido também como 12763-025
Preço (BRL)
-
Quantity:
500 mL

Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

  • Sera Category: Specialty
  • Specially tested for the ability to support the expansion and clonal enumeration (colony forming unit fibroblast assay) of mesenchymal stem cells
  • Endotoxin level: ≤50 EU/mL (levels routinely ≤10 EU/mL)
  • Hemoglobin level: ≤25 mg/dL
  • Country of Origin: United States
Especificações
Donor HerdNo
Endotoxin Concentration<50 EU/mL
Hemoglobin Concentration≤25 mg/dL
Purity or Quality GradeMS Cell-Qualified
Shipping ConditionDry Ice
SpeciesCattle/Bovine
Tested ForBovine Respiratory Syncytial Virus FA, Cytopathic Agents, Tetracycline Testing, Reovirus FA, Rabies FA, Hemoglobin (ng/dL), Blue Tongue Virus, Bovine Adenovirus FA, Bovine Parvovirus FA, Osmolality, Sp2 Performance Assay, Electrophoretic Pattern, Total Protein, Hemadsorbing Agents, Endotoxin (EU/mL), Mycoplasma, Sterility Testing (Bacteria and Fungi)
AgeFetal
Country of OriginUnited States
FormatBottle
Quantity500 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Unit SizeEach
Conteúdo e armazenamento
Storage conditions: ≤-10°C
Shipping conditions: Frozen

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

If my FBS arrives partially thawed, can I still use it?

Our studies have shown that short-term storage of thawed FBS at 4 degrees C for up to 28 days causes no decrease in growth or viability performance. FBS stored at 4 degrees C for longer periods of time should be evaluated on a case-by-case basis should this storage condition be necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Sometimes I notice flocculence in serum. What could it be?

Flocculence may appear in FBS for a variety of reasons. The most common reason is the denaturation of serum lipoproteins. You may observe fibrin, one of the clot-forming proteins present in serum, after the serum has been thawed. This should not affect product performance. To remove the flocculence, transfer the serum to sterile tubes and centrifuge the material briefly at 400 x g. Then filter the resulting supernant along with your media. Do not attempt to filter serum containing flocculence, it may clog filters.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does material precipitate in FBS upon storage in the refrigerator?

Gibco FBS is not pre-aged. When stored at 2 to 8 degrees C, the possibility exists for various proteins and lipoproteins in serum (e.g., cold agglutinins, fibrinogen, vitronectin, etc.) to aggregate, and form either perceptible material or observed turbidity. This should not affect serum performance. We recommend that you store FBS at -20 degrees C and avoid repeated freeze-thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.